Differential immunophenotype of circulating monocytes from pregnant women in response to viral ligands

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dc.catalogadorcrc
dc.contributor.authorFarías Jofré, Marcelo Enrique
dc.contributor.authorRomero, Roberto
dc.contributor.authorXu, Yi
dc.contributor.authorLevenson, Dustyn
dc.contributor.authorTao, Li
dc.contributor.authorKanninen, Tomi
dc.contributor.authorGalaz, Jose
dc.contributor.authorArenas-Hernandez, Marcia
dc.contributor.authorLiu, Zhenjie
dc.contributor.authorMiller, Derek
dc.contributor.authorBhatti, Gaurav
dc.contributor.authorSeyerle, Megan
dc.contributor.authorTarca, Adi L.
dc.contributor.authorGomez-Lopez, Nardhy
dc.date.accessioned2023-05-11T14:02:48Z
dc.date.available2023-05-11T14:02:48Z
dc.date.issued2023
dc.date.updated2023-05-07T00:02:40Z
dc.description.abstractBackground Viral infections during pregnancy can have deleterious effects on mothers and their offspring. Monocytes participate in the maternal host defense against invading viruses; however, whether pregnancy alters monocyte responses is still under investigation. Herein, we undertook a comprehensive in vitro study of peripheral monocytes to characterize the differences in phenotype and interferon release driven by viral ligands between pregnant and non-pregnant women. Methods Peripheral blood was collected from third-trimester pregnant (n = 20) or non-pregnant (n = 20, controls) women. Peripheral blood mononuclear cells were isolated and exposed to R848 (TLR7/TLR8 agonist), Gardiquimod (TLR7 agonist), Poly(I:C) (HMW) VacciGrade™ (TLR3 agonist), Poly(I:C) (HMW) LyoVec™ (RIG-I/MDA-5 agonist), or ODN2216 (TLR9 agonist) for 24 h. Cells and supernatants were collected for monocyte phenotyping and immunoassays to detect specific interferons, respectively. Results The proportions of classical (CD14hiCD16−), intermediate (CD14hiCD16+), non-classical (CD14loCD16+), and CD14loCD16− monocytes were differentially affected between pregnant and non-pregnant women in response to TLR3 stimulation. The proportions of pregnancy-derived monocytes expressing adhesion molecules (Basigin and PSGL-1) or the chemokine receptors CCR5 and CCR2 were diminished in response to TLR7/TLR8 stimulation, while the proportions of CCR5− monocytes were increased. Such differences were found to be primarily driven by TLR8 signaling, rather than TLR7. Moreover, the proportions of monocytes expressing the chemokine receptor CXCR1 were increased during pregnancy in response to poly(I:C) stimulation through TLR3, but not RIG-I/MDA-5. By contrast, pregnancy-specific changes in the monocyte response to TLR9 stimulation were not observed. Notably, the soluble interferon response to viral stimulation by mononuclear cells was not diminished in pregnancy. Conclusions Our data provide insight into the differential responsiveness of pregnancy-derived monocytes to ssRNA and dsRNA, mainly driven by TLR8 and membrane-bound TLR3, which may help to explain the increased susceptibility of pregnant women to adverse outcomes resulting from viral infection as observed during recent and historic pandemics.
dc.fechaingreso.objetodigital2023-05-07
dc.fuente.origenAutoarchivo
dc.identifier.citationarias-Jofre, M., Romero, R., Xu, Y. et al. Differential immunophenotype of circulating monocytes from pregnant women in response to viral ligands. BMC Pregnancy Childbirth 23, 323 (2023). https://doi.org/10.1186/s12884-023-05562-0
dc.identifier.doi10.1186/s12884-023-05562-0
dc.identifier.issn1471-2393
dc.identifier.urihttps://doi.org/10.1186/s12884-023-05562-0
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/66952
dc.identifier.wosidWOS:000982631500001
dc.information.autorucFacultad de Medicina;Farías Jofré, Marcelo Enrique; 0000-0003-0473-2295 ;12286
dc.information.autorucFacultad de Medicina ;Galaz, José ; 0000-0002-8160-8581;1034327
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final17
dc.pagina.inicio1
dc.revistaBMC Pregnancy and Childbirth
dc.rightsacceso abierto
dc.rights.holderThe Author(s)
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectVirus
dc.subjectInnate immunity
dc.subjectInfection
dc.subjectPregnancy
dc.subjectHuman
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDifferential immunophenotype of circulating monocytes from pregnant women in response to viral ligands
dc.typeartículo
dc.volumen23
sipa.codpersvinculados12286
sipa.codpersvinculados1034327
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