Lipopolysaccharide Activates Toll-Like Receptor 4 and Prevents Cardiac Fibroblast-to-Myofibroblast Differentiation
dc.contributor.author | Bolivar, Samir | |
dc.contributor.author | Santana, Roxana | |
dc.contributor.author | Ayala, Pedro | |
dc.contributor.author | Landaeta, Rodolfo | |
dc.contributor.author | Boza, Pia | |
dc.contributor.author | Humeres, Claudio | |
dc.contributor.author | Vivar, Raul | |
dc.contributor.author | Munoz, Claudia | |
dc.contributor.author | Pardo, Viviana | |
dc.contributor.author | Fernandez, Samuel | |
dc.contributor.author | Anfossi, Renatto | |
dc.contributor.author | Diaz Araya, Guillermo | |
dc.date.accessioned | 2024-01-10T13:50:00Z | |
dc.date.available | 2024-01-10T13:50:00Z | |
dc.date.issued | 2017 | |
dc.description.abstract | Bacterial lipopolysaccharide (LPS) is a known ligand of Toll-like receptor 4 (TLR4) which is expressed in cardiac fibroblasts (CF). Differentiation of CF to cardiac myofibroblasts (CMF) is induced by transforming growth factor-beta 1 (TGF-beta 1), increasing alpha-smooth muscle actin (alpha-SMA) expression. In endothelial cells, an antagonist effect between LPS-induced signaling and canonical TGF-beta 1 signaling was described; however, it has not been studied whether in CF and CMF the expression of alpha-SMA induced by TGF-beta 1 is antagonized by LPS and the mechanism involved. In adult rat CF and CMF, alpha-SMA, ERK1/2, Akt, NF-kappa beta, Smad3, and Smad7 protein levels were determined by western blot, TGF-beta isoforms by ELISA, and alpha-SMA stress fibers by immunocytochemistry. CF and CMF secrete the three TGF-beta isoforms, and the secretion levels of TGF-beta 2 was affected by LPS treatment. In CF, LPS treatment decreased the protein levels of alpha-SMA, and this effect was prevented by TAK-242 (TLR4 inhibitor) and LY294002 (Akt inhibitor), but not by BAY 11-7082 (NF-kappa beta inhibitor) and PD98059 (ERK1/2 inhibitor). TGF-beta 1 increased alpha-SMA protein levels in CF, and LPS prevented partially this effect. In addition, in CMF alpha-SMA protein levels were decreased by LPS treatment, which was abolished by TAK-242. Finally, in CF LPS decreased the p-Smad3 phosphorylation and increased the Smad7 protein levels. LPS treatment prevents the CF-to-CMF differentiation and reverses the CMF phenotype induced by TGF-beta 1, through decreasing p-Smad3 and increasing Smad7 protein levels. | |
dc.description.funder | FONDECYT | |
dc.description.funder | CONICYT | |
dc.fechaingreso.objetodigital | 2024-03-14 | |
dc.format.extent | 13 páginas | |
dc.fuente.origen | WOS | |
dc.identifier.doi | 10.1007/s12012-017-9404-4 | |
dc.identifier.eissn | 1559-0259 | |
dc.identifier.issn | 1530-7905 | |
dc.identifier.pubmedid | MEDLINE:28220374 | |
dc.identifier.uri | https://doi.org/10.1007/s12012-017-9404-4 | |
dc.identifier.uri | https://repositorio.uc.cl/handle/11534/79498 | |
dc.identifier.wosid | WOS:000411104600010 | |
dc.information.autoruc | Medicina; Ayala P;S/I;1013060 | |
dc.issue.numero | 4 | |
dc.language.iso | en | |
dc.nota.acceso | Contenido parcial | |
dc.pagina.final | 470 | |
dc.pagina.inicio | 458 | |
dc.publisher | HUMANA PRESS INC | |
dc.revista | CARDIOVASCULAR TOXICOLOGY | |
dc.rights | acceso restringido | |
dc.subject | Lipopolysaccharide | |
dc.subject | TLR4 | |
dc.subject | Cardiac fibroblast | |
dc.subject | alpha-SMA | |
dc.subject | MUSCLE ACTIN EXPRESSION | |
dc.subject | TGF-BETA RECEPTOR | |
dc.subject | EXTRACELLULAR-MATRIX | |
dc.subject | SMAD7 | |
dc.subject | CELLS | |
dc.subject | FIBROSIS | |
dc.subject | TLR4 | |
dc.subject | INTERACTS | |
dc.subject | MECHANISM | |
dc.subject | APOPTOSIS | |
dc.subject.ods | 03 Good Health and Well-being | |
dc.subject.odspa | 03 Salud y bienestar | |
dc.title | Lipopolysaccharide Activates Toll-Like Receptor 4 and Prevents Cardiac Fibroblast-to-Myofibroblast Differentiation | |
dc.type | artículo | |
dc.volumen | 17 | |
sipa.codpersvinculados | 1013060 | |
sipa.index | WOS | |
sipa.index | Scopus | |
sipa.trazabilidad | Carga SIPA;09-01-2024 |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- 2024-03-14. Lipopolysaccharide Activates Toll-Like Receptor 4 and Prevents Cardiac Fibroblast-to-Myofibroblast Differentiation.pdf
- Size:
- 123 KB
- Format:
- Adobe Portable Document Format
- Description: