Diversity of non-o157 shiga toxin-producing escherichia coli isolated from cattle from central and southern chile

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dc.contributor.authorDíaz L.
dc.contributor.authorGutierrez S.
dc.contributor.authorNavarrete P.
dc.contributor.authorReyes-Jara A.
dc.contributor.authorToro M.
dc.contributor.authorMoreno-Switt A.I.
dc.contributor.authorMoreno-Switt A.I.
dc.contributor.authorHervé L.P.
dc.contributor.authorHamilton-West C.
dc.contributor.authorHervé L.P.
dc.contributor.authorPadola N.L.
dc.contributor.authorNavarrete P.
dc.contributor.authorMeng J.
dc.contributor.authorGonzález-Escalona N.
dc.date.accessioned2024-01-10T13:10:09Z
dc.date.available2024-01-10T13:10:09Z
dc.date.issued2021
dc.description.abstract© 2021 by the authors. Licensee MDPI, Basel, Switzerland.Cattle are the main reservoir of Shiga toxin-producing Escherichia coli (STEC), one of the world’s most important foodborne pathogens. The pathogen causes severe human diseases and outbreaks. This study aimed to identify and characterize non-O157 STEC isolated from cattle feces from central and southern Chile. We analyzed 446 cattle fecal samples and isolated non-O157 STEC from 12.6% (56/446); a total of 93 different isolates were recovered. Most isolates displayed β-glucuronidase activity (96.8%; 90/93) and fermented sorbitol (86.0%; 80/93), whereas only 39.8% (37/93) were resistant to tellurite. A subgroup of 30 representative non-O157 STEC isolates was selected for whole-genome sequencing and bioinformatics analysis. In silico analysis showed that they grouped into 16 different sequence types and 17 serotypes; the serotypes most frequently identified were O116:H21 and O168:H8 (13% each). A single isolate of serotype O26:H11 was recovered. One isolate was resistant to tetracycline and carried resistance genes tet(A) and tet(R); no other isolate displayed antimicrobial resistance or carried antimicrobial resistance genes. The intimin gene (eae) was identified in 13.3% (4/30) of the genomes and 90% (27/30) carried the stx2 gene. A phylogenetic reconstruction demonstrated that the isolates clustered based on serotypes, independent of geographical origin. These results indicate that cattle in Chile carry a wide diversity of STEC potentially pathogenic for humans based on the presence of critical virulence genes.
dc.description.funderNational Committee for Science and Technology of Chile
dc.description.funderSantander Universidades
dc.description.funderU-Inicia
dc.description.funderCONICYT
dc.description.funderFONDECYT
dc.description.funderUniversidad de Chile
dc.description.funderMinistry of Economy, Development, and Tourism
dc.fechaingreso.objetodigital2024-05-23
dc.fuente.origenScopus
dc.identifier.doi10.3390/ani11082388
dc.identifier.eissn20762615
dc.identifier.issn20762615
dc.identifier.pubmedidMEDLINE:34438845
dc.identifier.scopusidSCOPUS_ID:85112382281
dc.identifier.urihttps://doi.org/10.3390/ani11082388
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77787
dc.identifier.wosidWOS:000688691000001
dc.information.autorucFacultad de Agronomía e Ingeniería Forestal; Moreno Switt, Andrea Isabel; S/I; 1147061
dc.issue.numero2388
dc.language.isoen
dc.nota.accesoContenido completo
dc.publisherMDPI AG
dc.revistaAnimals
dc.rightsacceso abierto
dc.subjectCattle
dc.subjectNon-O157
dc.subjectSTEC
dc.subjectStx genes
dc.subjectVirulence genes
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDiversity of non-o157 shiga toxin-producing escherichia coli isolated from cattle from central and southern chile
dc.typeartículo
dc.volumen11
sipa.codpersvinculados1147061
sipa.indexWos
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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