Distribution and Activity of Sulfur-Metabolizing Bacteria along the Temperature Gradient in Phototrophic Mats of the Chilean Hot Spring Porcelana

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dc.article.number1803
dc.catalogadorgjm
dc.contributor.authorKonrad, Ricardo
dc.contributor.authorVergara Barros, Pablo
dc.contributor.authorAlcorta Loyola, Jaime Andrés
dc.contributor.authorAlcamán Arias, María E.
dc.contributor.authorLevicán, Gloria
dc.contributor.authorRidley, Christina
dc.contributor.authorDiez Moreno, Beatriz
dc.date.accessioned2023-07-17T17:10:54Z
dc.date.available2023-07-17T17:10:54Z
dc.date.issued2023
dc.description.abstractIn terrestrial hot springs, some members of the microbial mat community utilize sulfur chemical species for reduction and oxidization metabolism. In this study, the diversity and activity of sulfur-metabolizing bacteria were evaluated along a temperature gradient (48–69 °C) in non-acidic phototrophic mats of the Porcelana hot spring (Northern Patagonia, Chile) using complementary meta-omic methodologies and specific amplification of the aprA (APS reductase) and soxB (thiosulfohydrolase) genes. Overall, the key players in sulfur metabolism varied mostly in abundance along the temperature gradient, which is relevant for evaluating the possible implications of microorganisms associated with sulfur cycling under the current global climate change scenario. Our results strongly suggest that sulfate reduction occurs throughout the whole temperature gradient, being supported by different taxa depending on temperature. Assimilative sulfate reduction is the most relevant pathway in terms of taxonomic abundance and activity, whereas the sulfur-oxidizing system (Sox) is likely to be more diverse at low rather than at high temperatures. Members of the phylum Chloroflexota showed higher sulfur cycle-related transcriptional activity at 66 °C, with a potential contribution to sulfate reduction and oxidation to thiosulfate. In contrast, at the lowest temperature (48 °C), Burkholderiales and Acetobacterales (both Pseudomonadota, also known as Proteobacteria) showed a higher contribution to dissimilative sulfate reduction/oxidation as well as to thiosulfate metabolism. Cyanobacteriota and Planctomycetota were especially active in assimilatory sulfate reduction. Analysis of the aprA and soxB genes pointed to members of the order Burkholderiales (Gammaproteobacteria) as the most dominant and active along the temperature gradient for these genes. Changes in the diversity and activity of different sulfur-metabolizing bacteria in photoautotrophic microbial mats along a temperature gradient revealed their important role in hot spring environments, especially the main primary producers (Chloroflexota/Cyanobacteriota) and diazotrophs (Cyanobacteriota), showing that carbon, nitrogen, and sulfur cycles are highly linked in these extreme systems.
dc.fechaingreso.objetodigital2023-07-17
dc.format.extent19 páginas
dc.fuente.origenORCID
dc.identifier.doi10.3390/microorganisms11071803
dc.identifier.eissn2076-2607
dc.identifier.urihttps://doi.org/10.3390/microorganisms11071803
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/74185
dc.information.autorucFacultad de Ciencias Biológicas; Vergara Barros, Pablo; 0000-0001-9565-2711; 187013
dc.information.autorucFacultad de Ciencias Biológicas; Alcorta Loyola, Jaime Andrés; 0000-0001-7662-239X; 187040
dc.information.autorucFacultad de Ciencias Biológicas; Diez Moreno, Beatriz; 0000-0002-9371-8083; 1009001
dc.issue.numero7
dc.language.isoen
dc.nota.accesoContenido completo
dc.revistaMicroorganisms
dc.rightsacceso abierto
dc.subjectSulfur oxidation/reduction
dc.subjectsoxB
dc.subjectaprA
dc.subjectPhototrophic hot spring mat
dc.subjectMetagenomics
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.subject.ods13 Climate action
dc.subject.odspa13 Acción por el clima
dc.titleDistribution and Activity of Sulfur-Metabolizing Bacteria along the Temperature Gradient in Phototrophic Mats of the Chilean Hot Spring Porcelana
dc.typeartículo
dc.volumen11
sipa.codpersvinculados187013
sipa.codpersvinculados187040
sipa.codpersvinculados1009001
sipa.trazabilidadORCID;2023-07-17
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