An Open One-Step RT-qPCR for SARS-CoV-2 detection

Simple item page
dc.contributor.authorCerda, Ariel
dc.contributor.authorRivera, Maira
dc.contributor.authorArmijo, Grace
dc.contributor.authorIbarra-Henriquez, Catalina
dc.contributor.authorReyes, Javiera
dc.contributor.authorBlazquez-Sanchez, Paula
dc.contributor.authorAviles, Javiera
dc.contributor.authorArce, Anibal
dc.contributor.authorSeguel, Aldo
dc.contributor.authorBrown, Alexander J.
dc.contributor.authorVasquez, Yesseny
dc.contributor.authorCortez-San Martin, Marcelo
dc.contributor.authorCubillos, Francisco A.
dc.contributor.authorGarcia, Patricia
dc.contributor.authorFerres, Marcela
dc.contributor.authorRamirez-Sarmiento, Cesar A.
dc.contributor.authorFederici, Fernan
dc.contributor.authorGutierrez, Rodrigo A.
dc.date.accessioned2025-01-20T17:08:50Z
dc.date.available2025-01-20T17:08:50Z
dc.date.issued2024
dc.description.abstractThe COVID-19 pandemic has resulted in millions of deaths globally, and while several diagnostic systems were proposed, real-time reverse transcription polymerase chain reaction (RT-PCR) remains the gold standard. However, diagnostic reagents, including enzymes used in RT-PCR, are subject to centralized production models and intellectual property restrictions, which present a challenge for less developed countries. With the aim of generating a standardized One-Step open RT-qPCR protocol to detect SARS-CoV-2 RNA in clinical samples, we purified and tested recombinant enzymes and a non-proprietary buffer. The protocol utilized M-MLV RT and Taq DNA pol enzymes to perform a Taqman probe-based assay. Synthetic RNA samples were used to validate the One-Step RT-qPCR components, demonstrating sensitivity comparable to a commercial kit routinely employed in clinical settings for patient diagnosis. Further evaluation on 40 clinical samples (20 positive and 20 negative) confirmed its comparable diagnostic accuracy. This study represents a proof of concept for an open approach to developing diagnostic kits for viral infections and diseases, which could provide a cost-effective and accessible solution for less developed countries.
dc.fuente.origenWOS
dc.identifier.doi10.1371/journal.pone.0297081
dc.identifier.issn1932-6203
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0297081
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/90988
dc.identifier.wosidWOS:001158471300068
dc.issue.numero1
dc.language.isoen
dc.revistaPlos one
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleAn Open One-Step RT-qPCR for SARS-CoV-2 detection
dc.typeartículo
dc.volumen19
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
An Open One-Step RT-qPCR for SARS-CoV-2.pdf
Size:
1.11 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Artículos de revistas