<i>In vitro</i> regeneration of <i>Gomortega keule</i> (<i>Gomortegaceae</i>), a Chilean endemic tree in danger of extinction
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Date
2005
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Abstract
Axillary buds of nodal sections from 2-year old trees of Gomortega keule (Mol.) Baillon, 'Queule', 'Keule', (Family: Gomorte gaceae) were induced to sprout and developed many shoots in presence of 0.54 mu M naphthaleneacetic acid (NAA) and 4.44 mu M benzylaminopurine (BA) in a liquid WP-medium (LLOYD and McCowN 1980) within a period of 2-3 weeks. After transfer to a medium containing 22.2 mu M BA, each new single node section of about 0.5 cm initiated numerous adventitious shoots; alternatively, roots were formed when these explants were transferred to a medium containing 2.95, 12.3 or 24.6 mu M indolebutyric acid (IBA). Root formation took place in presence of 24.6 mu M IBA in approx. 46 % of the sub-cultured nodal segments after a period of 3 months, leading to plandets. Regeneration response was restricted to the sprouts of axillary buds; other explant-types tested, i.e. petioles, leaf, and internodal sections showed callus formation only and no other responses.
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Gomortega keule, endangered species, organogenesis, in vitro regeneration