Characterization of hepatic fatty acids using magnetic resonance spectroscopy for the assessment of treatment response to metformin in an eNOS−/− mouse model of metabolic nonalcoholic fatty liver disease/nonalcoholic steatohepatitis

Simple item page
dc.catalogadorgjm
dc.contributor.authorLavin, Begoña
dc.contributor.authorEykyn, Thomas
dc.contributor.authorPhinikaridou, Alkystis
dc.contributor.authorXavier, Aline
dc.contributor.authorKumar, Shravan
dc.contributor.authorBuqué, Xabier
dc.contributor.authorAspichueta, Patricia
dc.contributor.authorSing-Long C., Carlos A.
dc.contributor.authorArrese, Marco
dc.contributor.authorBotnar, René Michael
dc.contributor.authorAndía Kohnenkampf, Marcelo Edgardo
dc.date.accessioned2024-01-30T15:23:29Z
dc.date.available2024-01-30T15:23:29Z
dc.date.issued2023
dc.description.abstractNonalcoholic fatty liver disease (NAFLD) is the leading cause of chronic liver disease worldwide. Liver biopsy remains the gold standard for diagnosis and staging of disease. There is a clinical need for noninvasive diagnostic tools for risk stratification, follow-up, and monitoring treatment response that are currently lacking, as well as preclinical models that recapitulate the etiology of the human condition. We have characterized the progression of NAFLD in eNOS−/− mice fed a high fat diet (HFD) using noninvasive Dixon-based magnetic resonance imaging and single voxel STEAM spectroscopy-based protocols to measure liver fat fraction at 3 T. After 8 weeks of diet intervention, eNOS−/− mice exhibited significant accumulation of intra-abdominal and liver fat compared with control mice. Liver fat fraction measured by 1H-MRS in vivo showed a good correlation with the NAFLD activity score measured by histology. Treatment of HFD-fed NOS3−/− mice with metformin showed significantly reduced liver fat fraction and altered hepatic lipidomic profile compared with untreated mice. Our results show the potential of in vivo liver MRI and 1H-MRS to noninvasively diagnose and stage the progression of NAFLD and to monitor treatment response in an eNOS−/− murine model that represents the classic NAFLD phenotype associated with metabolic syndrome.
dc.fechaingreso.objetodigital2024-06-06
dc.fuente.origenORCID
dc.identifier.doi10.1002/nbm.4932
dc.identifier.urihttps://kclpure.kcl.ac.uk/portal/en/publications/4fabde41-2c0d-438e-8cac-5448d00666fd
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/81060
dc.identifier.wosidWOS:000969102500001
dc.information.autorucEscuela de Ingeniería; Sing-Long C., Carlos A.; 0000-0002-2533-2509; 126170
dc.information.autorucEscuela de Medicina; Arrese, Marco; 0000-0002-0499-4191; 76095
dc.information.autorucEscuela de Ingeniería; Botnar, René Michael; 0000-0002-9447-4367; 1015313
dc.information.autorucEscuela de Medicina; Andía Kohnenkampf, Marcelo Edgardo; 0000-0002-1251-5832; 90691
dc.language.isoen
dc.nota.accesoContenido completo
dc.rightsacceso abierto
dc.subjecteNOS-/-
dc.subjectMetformin
dc.subjectMRI
dc.subjectNAFLD
dc.subjectThin-layer chromatography
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCharacterization of hepatic fatty acids using magnetic resonance spectroscopy for the assessment of treatment response to metformin in an eNOS−/− mouse model of metabolic nonalcoholic fatty liver disease/nonalcoholic steatohepatitis
dc.typeartículo
sipa.codpersvinculados126170
sipa.codpersvinculados76095
sipa.codpersvinculados1015313
sipa.codpersvinculados90691
sipa.trazabilidadORCID;2024-01-15
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Characterization of hepatic fatty acids using magnetic resonance spectroscopy.pdf
Size:
2.75 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Artículos de revistas