Rapid molecular detection of pulmonary tuberculosis in HIV-infected patients in Santiago, Chile

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dc.catalogadorpva
dc.contributor.authorBalcells Marty, María Elvira
dc.contributor.authorGarcía Cañete, Patricia
dc.contributor.authorChanqueo, L.
dc.contributor.authorBahamondes, L.
dc.contributor.authorLasso, M.
dc.contributor.authorGallardo, A. M.
dc.contributor.authorCifuentes, Lorena
dc.date.accessioned2023-11-08T19:16:58Z
dc.date.available2023-11-08T19:16:58Z
dc.date.issued2012
dc.description.abstractSETTING: Santiago, Chile, has a mean annual tuberculosis (TB) rate of 13 per 100 000 population; however, TB incidence in human immunodeficiency virus (HIV) infected individuals is at least 20 times higher. OBJECTIVE: To assess the accuracy of rapid molecular testing for pulmonary TB (PTB) detection in routine care in HIV-infected patients. DESIGN: Cross-sectional study, conducted prospectively in five hospitals between March 2010 and June 2011. HIV-positive subjects with suspected PTB provided sputum or mouth wash samples that were directly processed for acid-fast smear, mycobacterial cultures and Xpert® MTB/RIF. Positive test results were reported on the same day. RESULTS: We enrolled 166 subjects into the study; 50.6% provided two sputum samples, 33.1% only one sputum sample and 16.3% a mouth wash sample. The prevalence of TB was 8.1% (13/160). Diagnostic sensitivity increased from 66.7% (95%CI 39.1–86.2) for acid-fast smear to 91.7% (95%CI 64.6–98.5) for Xpert MTB/RIF, with comparable specificity at 98.6% (146/148, 95%CI 95.2–99.6) and 99.3% (147/148, 95%CI 96.3–99.9). Xpert MTB/RIF allowed early detection of rifampicin resistance in 16.6% of cases, with rapid adjustment to multidrug-resistant treatment. CONCLUSION: Xpert MTB/RIF provided earlier TB diagnosis in 25% more cases than acid-fast smear alone. Its implementation should be considered for TB diagnosis in HIV-positive patients even outside TB-endemic areas.
dc.description.funderNational Scientific and Techonological Research Comission of Chile (Comision Nacional de Investigacion Cientifica y Tecnologica-CONYCIT, FONIS)
dc.fechaingreso.objetodigital2023-11-08
dc.format.extent6 páginas
dc.fuente.origenWOS
dc.identifier.doi10.5588/ijtld.12.0156
dc.identifier.eissn1815-7920
dc.identifier.issn1027-3719
dc.identifier.pubmedidMEDLINE:22863872
dc.identifier.urihttps://doi.org/10.5588/ijtld.12.0156
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75256
dc.identifier.wosidWOS:000310413100013
dc.information.autorucEscuela de Medicina; Balcells Marty, María Elvira; 0000-0002-7223-9665; 7462
dc.information.autorucEscuela de Medicina; García Cañete, Patricia; 0000-0002-5983-2392; 73909
dc.information.autorucEscuela de Medicina; Cifuentes, Lorena; 0000-0002-9749-9201; 70068
dc.issue.numero10
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final1353
dc.pagina.inicio1349
dc.publisherInternational Union Against Tuberculosis and Lung Disease
dc.revistaThe International Journal of Tuberculosis and Lung Diseasees_ES
dc.rightsacceso abierto
dc.subjectTuberculosises_ES
dc.subjectHIVes_ES
dc.subjectDiagnosises_ES
dc.subjectXpert (R) MTB/RIFes_ES
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleRapid molecular detection of pulmonary tuberculosis in HIV-infected patients in Santiago, Chilees_ES
dc.typeartículo
dc.volumen16
sipa.codpersvinculados7462
sipa.codpersvinculados73909
sipa.codpersvinculados70068
sipa.indexISI
sipa.indexScopus
sipa.trazabilidadWOS;05-06-2021
sipa.trazabilidadORCID;2023-11-06
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