Isolation of the three grape sub-lineages of B-class MADS-box <i>TM6,</i> <i>PISTILLATA</i> and <i>APETALA3</i> genes which are differentially expressed during flower and fruit development

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dc.contributor.authorPoupin, Maria Josefina
dc.contributor.authorFederici, Fernan
dc.contributor.authorMedina, Consuelo
dc.contributor.authorMatus, Jose Tomas
dc.contributor.authorTimmermann, Tania
dc.contributor.authorArce-Johnson, Patricio
dc.date.accessioned2025-01-21T01:05:18Z
dc.date.available2025-01-21T01:05:18Z
dc.date.issued2007
dc.description.abstractThe B class of MADS-box floral homeotic genes specifies petal and stamen identity in angiosperms. While this group is one of the most studied in herbaceous plant species, it has remained largely uncharacterized in woody species such as grapevine. Although the B class PI/GLO and AP3/DEF clades have been extensively characterized in model species, the role of the TM6 subgroup within the AP3 clade is not completely understood, since it is absent in Arabidopsis thaliana. In this study, the coding regions of VvTM6 and VvAP3 and the genomic sequence of VvPI, were cloned. VvPI and AtPI were confirmed to be functional homologues by means of complementation of the pi Arabidopsis mutant. Expression analysis revealed that VvPI and VvAP3 transcripts are restricted almost exclusively to inflorescences, although VvPI was detected at low levels in leaves and roots. VvTM6 expresses throughout the plant, with higher levels in flowers and berries. A detailed chronological study of grape flower progression by light microscopy and temporal expression analysis throughout early and late developmental stages, revealed that VvPI expression increases during pollen maturation and decreases between the events of pollination and fertilization, before the cap fall. On the other hand, VvTM6 is expressed in the last stage of anther development. Specific expression of VvAP3 and VvPI was detected in petals and stamens within the flower, while VvTM6 was also expressed in carpels. Moreover, this work provides the first evidence for expression of a TM6-like gene throughout fruit growth and ripening. Even if these genes belong to the same genetic class they could act in different periods and/or tissues during reproductive organ development. (C) 2007 Elsevier B.V. All rights reserved.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.gene.2007.08.005
dc.identifier.eissn1879-0038
dc.identifier.issn0378-1119
dc.identifier.urihttps://doi.org/10.1016/j.gene.2007.08.005
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95887
dc.identifier.wosidWOS:000251074300002
dc.issue.numero1-2
dc.language.isoen
dc.pagina.final24
dc.pagina.inicio10
dc.revistaGene
dc.rightsacceso restringido
dc.subjectABC model
dc.subjectVitis vinifera
dc.subjectflowering
dc.subjectberry
dc.subjectquantitative RT-PCR
dc.subjectArabidopsis mutant complementation
dc.subjectAP3
dc.subjectPI
dc.subjectgrape genome sequence
dc.subjectgrape
dc.subjectflower
dc.subject.ods02 Zero Hunger
dc.subject.ods13 Climate Action
dc.subject.odspa02 Hambre cero
dc.subject.odspa13 Acción por el clima
dc.titleIsolation of the three grape sub-lineages of B-class MADS-box <i>TM6,</i> <i>PISTILLATA</i> and <i>APETALA3</i> genes which are differentially expressed during flower and fruit development
dc.typeartículo
dc.volumen404
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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