Acetyl xylan esterase II from <i>Penicillium purpurogenum</i> is similar to an esterase from <i>Trichoderma ressei</i> but lacks a cellulose binding domain

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dc.contributor.authorGutiérrez, R
dc.contributor.authorCederlund, E
dc.contributor.authorHjelmqvist, L
dc.contributor.authorPeirano, A
dc.contributor.authorHerrera, F
dc.contributor.authorGhosh, D
dc.contributor.authorDuax, W
dc.contributor.authorJörnvall, H
dc.contributor.authorEyzaguirre, J
dc.date.accessioned2025-01-21T01:32:50Z
dc.date.available2025-01-21T01:32:50Z
dc.date.issued1998
dc.description.abstractPenicillium purpurogenum produces at least two acetyl xylan esterases (AXE I and LI). The AXE II cDNA, genomic DNA and mature protein sequences were determined and show that the axe 2 gene contains two introns, that the primary translation product has a signal peptide of 27 residues, and that the mature protein has 207 residues, The sequence is similar to the catalytic domain of AXE I from Trichoderma reesei (67% residue identity) and putative active site residues are conserved, but the Penicillium enzyme lacks the linker and cellulose binding domain, thus explaining why it does not bind cellulose in contrast to the Tricoderma enzyme. These results point to a possible common ancestor gene for the active site domain, while the linker and the binding domain may have been added to the Trichoderma esterase by gene fusion. (C) 1998 Federation of European Biochemical Societies.
dc.fuente.origenWOS
dc.identifier.issn0014-5793
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97328
dc.identifier.wosidWOS:000072148800008
dc.issue.numero1
dc.language.isoen
dc.pagina.final38
dc.pagina.inicio35
dc.revistaFebs letters
dc.rightsacceso restringido
dc.subjectacetyl xylan esterase
dc.subjectcellulose binding domain
dc.subjectPenicillium purpurogenum
dc.subjectgene fusion
dc.subject.ods12 Responsible Consumption and Production
dc.subject.ods07 Affordable and Clean Energy
dc.subject.odspa12 Producción y consumo responsable
dc.subject.odspa07 Energía asequible y no contaminante
dc.titleAcetyl xylan esterase II from <i>Penicillium purpurogenum</i> is similar to an esterase from <i>Trichoderma ressei</i> but lacks a cellulose binding domain
dc.typeartículo
dc.volumen423
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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