TGA class II transcription factors are essential to restrict oxidative stress in response to UV-B stress in Arabidopsis

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dc.catalogadorgjm
dc.contributor.authorHerrera Vásquez, Ariel Esteban
dc.contributor.authorFonseca Cárdenas, Alejandro Alfredo
dc.contributor.authorUgalde Valdivia, José Manuel
dc.contributor.authorLamig Giannini, Liliana Andrea
dc.contributor.authorSeguel Avello, Aldo Luis
dc.contributor.authorMoyano Yugovic, Tomás Custodio
dc.contributor.authorGutiérrez Ilabaca, Rodrigo Antonio
dc.contributor.authorSalinas, Paula
dc.contributor.authorVidal, Elena A.
dc.contributor.authorHoluigue Barros, Loreto
dc.date.accessioned2024-01-30T15:23:29Z
dc.date.available2024-01-30T15:23:29Z
dc.date.issued2021
dc.description.abstractPlants possess a robust metabolic network for sensing and controlling reactive oxygen species (ROS) levels upon stress conditions. Evidence shown here supports a role for TGA class II transcription factors as critical regulators of genes controlling ROS levels in the tolerance response to UV-B stress in Arabidopsis. First, tga256 mutant plants showed reduced capacity to scavenge H2O2 and restrict oxidative damage in response to UV-B, and also to methylviologen-induced photooxidative stress. The TGA2 transgene (tga256/TGA2 plants) complemented these phenotypes. Second, RNAseq followed by clustering and Gene Ontology term analyses indicate that TGA2/5/6 positively control the UV-B-induced expression of a group of genes with oxidoreductase, glutathione transferase, and glucosyltransferase activities, such as members of the glutathione S-transferase Tau subfamily (GSTU), which encodes peroxide-scavenging enzymes. Accordingly, increased glutathione peroxidase activity triggered by UV-B was impaired in tga256 mutants. Third, the function of TGA2/5/6 as transcriptional activators of GSTU genes in the UV-B response was confirmed for GSTU7, GSTU8, and GSTU25, using quantitative reverse transcription–PCR and ChIP analyses. Fourth, expression of the GSTU7 transgene complemented the UV-B-susceptible phenotype of tga256 mutant plants. Together, this evidence indicates that TGA2/5/6 factors are key regulators of the antioxidant/detoxifying response to an abiotic stress such as UV-B light overexposure.
dc.fechaingreso.objetodigital2024-04-02
dc.fuente.origenORCID
dc.identifier.doi10.1093/jxb/eraa534
dc.identifier.urihttps://doi.org/10.1093/jxb/eraa534
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/81056
dc.identifier.wosidWOS:000637289800032
dc.information.autorucFacultad de Ciencias Biológicas; Herrera Vásquez, Ariel Esteban; 0000-0003-4313-8475; 142395
dc.information.autorucFacultad de Ciencias Biológicas; Fonseca Cárdenas, Alejandro Alfredo; S/I; 1031204
dc.information.autorucFacultad de Ciencias Biológicas; Ugalde Valdivia, José Manuel; S/I; 217895
dc.information.autorucFacultad de Ciencias Biológicas; Lamig Giannini, Liliana Andrea; S/I; 221018
dc.information.autorucFacultad de Ciencias Biológicas; Seguel Avello, Aldo Luis; S/I; 163573
dc.information.autorucFacultad de Ciencias Biológicas; Moyano Yugovic, Tomás Custodio; S/I; 149778
dc.information.autorucFacultad de Ciencias Biológicas; Gutiérrez Ilabaca, Rodrigo Antonio; 0000-0002-5961-5005; 86782
dc.information.autorucFacultad de Ciencias Biológicas; Holuigue Barros, Loreto; S/I; 50349
dc.language.isoen
dc.nota.accesoContenido completo
dc.rightsacceso abierto
dc.subjectArabidopsis
dc.subjectGlutathione S-transferase
dc.subjectGSTU7
dc.subjectPhotooxidative stress
dc.subjectRedox signaling
dc.subjectROS
dc.subjectTGA class II
dc.subjectTGA2
dc.subjectTranscription factor
dc.subjectUV-B stress
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.subject.ods02 Zero hunger
dc.subject.odspa02 Hambre cero
dc.titleTGA class II transcription factors are essential to restrict oxidative stress in response to UV-B stress in Arabidopsis
dc.typeartículo
sipa.codpersvinculados142395
sipa.codpersvinculados1031204
sipa.codpersvinculados217895
sipa.codpersvinculados221018
sipa.codpersvinculados163573
sipa.codpersvinculados149778
sipa.codpersvinculados86782
sipa.codpersvinculados50349
sipa.trazabilidadORCID;2024-01-15
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