Estrogen and luminal stimulation of rat uterine kallikrein

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dc.contributor.authorCorthorn, J
dc.contributor.authorFigueroa, C
dc.contributor.authorValdes, G
dc.date.accessioned2024-01-10T12:05:18Z
dc.date.available2024-01-10T12:05:18Z
dc.date.issued1997
dc.description.abstractTo understand the regulation of rat uterine kallikrein, we evaluated its variations in animals that had been ovariectomized and supplemented with estradiol or progesterone, in pseudopregnant animals intraluminally oil-stimulated or unstimulated, and in unilaterally pregnant animals. The content of kallikrein, determined by an RIA highly specific for rK1 (true tissue kallikrein), rose in ovariectomized rats with estradiol supplementation (0.28 +/- 0.03 to 0.44 +/- 0.05 ng/mg) and decreased with progesterone (0.13 +/- 0.02 ng/mg; n = 15; p < 0.001). Kallikrein content rose from Day 1 of pseudopregnancy (PP1) to a maximum on PP7 (0.18 +/- 0.01 to 0.39 +/- 0.04 ng/mg protein; n = 36; p < 0.001). On PP7 with unilateral oil intraluminal stimulation, the decidualized horn had higher kallikrein content than did the contralateral (0.98 +/- 0.09 vs. 0.35 +/- 0.05 ng/mg protein; n = 7; p < 0.001). Immunocytochemistry revealed that mainly rK1 is localized in the luminal and glandular epithelium, and it increased in the stimulated horn. In the unilaterally pregnant rat on Day 7, the fertile horn had a higher kallikrein content than its contralateral control (0.71 +/- 0.07 vs. 0.37 +/- 0.03 ng/mg protein, pt 0.001; n = 8), as well as a higher kininogenase activity (239 +/- 34.3 vs. 83.5 +/- 7.9 ng bradykinin(BK)/h per horn, p < 0.003; and 945 +/- 90 vs. 585 +/- 40 ng 8K/h per gram tissue, p < 0.002; n = 6). These results indicate that estrogen stimulates, whereas progesterone inhibits, kallikrein production, and that hormonal regulation is overridden by intraluminal stimulation, thus associating the enzyme with decidualization.
dc.fechaingreso.objetodigital2024-04-30
dc.format.extent7 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1095/biolreprod56.6.1432
dc.identifier.eissn1529-7268
dc.identifier.issn0006-3363
dc.identifier.pubmedidMEDLINE:9166695
dc.identifier.urihttps://doi.org/10.1095/biolreprod56.6.1432
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75983
dc.identifier.wosidWOS:A1997XA16700010
dc.information.autorucMedicina;Valdés G;S/I;98654
dc.issue.numero6
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final1438
dc.pagina.inicio1432
dc.publisherOXFORD UNIV PRESS INC
dc.revistaBIOLOGY OF REPRODUCTION
dc.rightsacceso restringido
dc.subjectPROGESTERONE-RECEPTOR
dc.subjectIMMUNOHISTOCHEMICAL LOCALIZATION
dc.subjectCELL-PROLIFERATION
dc.subjectTISSUE KALLIKREIN
dc.subjectESTERASE-B
dc.subjectRK2 TONIN
dc.subjectEXPRESSION
dc.subjectESTRADIOL
dc.subjectFAMILY
dc.subjectUTERUS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleEstrogen and luminal stimulation of rat uterine kallikrein
dc.typeartículo
dc.volumen56
sipa.codpersvinculados98654
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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