<i>In</i> <i>Vivo </i>and <i>in</i> <i>vitro</i> antitumor activity of tomatine in hepatocellular carcinoma

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dc.contributor.authorEcheverria, Cesar
dc.contributor.authorMartin, Aldo
dc.contributor.authorSimon, Felipe
dc.contributor.authorSalas, Cristian O.
dc.contributor.authorNazal, Mariajesus
dc.contributor.authorVarela, Diego
dc.contributor.authorPerez-Castro, Ramon A.
dc.contributor.authorSantibanez, Juan F.
dc.contributor.authorValdes-Valdes, Ricardo O.
dc.contributor.authorForero-Doria, Oscar
dc.contributor.authorEcheverria, Javier
dc.date.accessioned2025-01-20T21:02:24Z
dc.date.available2025-01-20T21:02:24Z
dc.date.issued2022
dc.description.abstractBackground: There is abundant ethnopharmacological evidence the uses of regarding Solanum species as antitumor and anticancer agents. Glycoalkaloids are among the molecules with antiproliferative activity reported in these species. Purpose: To evaluate the anticancer effect of the Solanum glycoalkaloid tomatine in hepatocellular carcinoma (HCC) in vitro (HepG2 cells) and in vivo models. Methods: The resazurin reduction assay was performed to detect the effect of tomatine on cell viability in human HepG2 cell lines. Programmed cell death was investigated by means of cellular apoptosis assays using Annexin V. The expression of cancer related proteins was detected by Western blotting (WB). Reactive oxygen species (ROS) and calcium were determined by 2,7-dichlorodihydrofluorescein diacetate and Fluo-4, respectively. Intrahepatic HepG2 xenograft mouse model was used to elucidate the effect of tomatine on tumor growth in vivo. Results and Discussion: Tomatine reduced HepG2 cell viability and induced the early apoptosis phase of cell death, consistently with caspase-3, -7, Bcl-2 family, and P53 proteins activation. Furthermore, tomatine increased intracellular ROS and cytosolic Ca+2 levels. Moreover, the NSG mouse xenograft model showed that treating mice with tomatine inhibited HepG2 tumor growth. Conclusion: Tomatine inhibits in vitro and in vivo HCC tumorigenesis in part via modulation of p53, Ca+2, and ROS signalling. Thus, the results suggest the potential cancer therapeutic use of tomatine in HCC patients.
dc.fuente.origenWOS
dc.identifier.doi10.3389/fphar.2022.1003264
dc.identifier.eissn1663-9812
dc.identifier.urihttps://doi.org/10.3389/fphar.2022.1003264
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93032
dc.identifier.wosidWOS:000859220700001
dc.language.isoen
dc.revistaFrontiers in pharmacology
dc.rightsacceso restringido
dc.subjectSolanum glycoalkaloids
dc.subjecttomatine
dc.subjectantitumoral activity
dc.subjecthepatocellular carcinoma
dc.subjectapoptosis
dc.subjectcaspase pathways
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.title<i>In</i> <i>Vivo </i>and <i>in</i> <i>vitro</i> antitumor activity of tomatine in hepatocellular carcinoma
dc.typeartículo
dc.volumen13
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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