The p75 neurotrophin receptor evades the endolysosomal route in neuronal cells, favouring multivesicular bodies specialised for exosomal release

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dc.contributor.authorEscudero, Claudia A.
dc.contributor.authorLazo, Oscal M.
dc.contributor.authorGalleguillos, Carolina
dc.contributor.authorParraguez, Jose I.
dc.contributor.authorLopez-Verrilli, Maria A.
dc.contributor.authorCabeza, Carolina
dc.contributor.authorLeon, Luisa
dc.contributor.authorSaeed, Uzma
dc.contributor.authorRetamal, Claudio
dc.contributor.authorGonzalez, Alfonso
dc.contributor.authorMarzolo, Maria-Paz
dc.contributor.authorCarter, Bruce D.
dc.contributor.authorCourt, Felipe A.
dc.contributor.authorBronfman, Francisca C.
dc.date.accessioned2025-01-23T21:46:29Z
dc.date.available2025-01-23T21:46:29Z
dc.date.issued2014
dc.description.abstractThe p75 neurotrophin receptor (p75, also known as NGFR) is a multifaceted signalling receptor that regulates neuronal physiology, including neurite outgrowth, and survival and death decisions. A key cellular aspect regulating neurotrophin signalling is the intracellular trafficking of their receptors; however, the post-endocytic trafficking of p75 is poorly defined. We used sympathetic neurons and rat PC12 cells to study the mechanism of internalisation and post-endocytic trafficking of p75. We found that p75 internalisation depended on the clathrin adaptor protein AP2 and on dynamin. More surprisingly, p75 evaded the lysosomal route at the level of the early endosome, instead accumulating in two different types of endosomes, Rab11-positive endosomes and multivesicular bodies (MVBs) positive for CD63, a marker of the exosomal pathway. Consistently, depolarisation by KCl induced the liberation of previously endocytosed full-length p75 into the extracellular medium in exosomes. Thus, p75 defines a subpopulation of MVBs that does not mature to lysosomes and is available for exosomal release by neuronal cells.
dc.fuente.origenWOS
dc.identifier.doi10.1242/jcs.141754
dc.identifier.eissn1477-9137
dc.identifier.issn0021-9533
dc.identifier.urihttps://doi.org/10.1242/jcs.141754
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101726
dc.identifier.wosidWOS:000335814800010
dc.issue.numero9
dc.language.isoen
dc.pagina.final1979
dc.pagina.inicio1966
dc.revistaJournal of cell science
dc.rightsacceso restringido
dc.subjectMVBs
dc.subjectTraffic
dc.subjectEndocytosis
dc.subjectNeurotrophins
dc.subjectp75
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleThe p75 neurotrophin receptor evades the endolysosomal route in neuronal cells, favouring multivesicular bodies specialised for exosomal release
dc.typeartículo
dc.volumen127
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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