Gonadotrophin-releasing hormone antagonists inhibit sperm binding to the human zona pellucida

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dc.contributor.authorMorales, P
dc.contributor.authorKerr, B
dc.contributor.authorOliva, C
dc.contributor.authorPizarro, E
dc.contributor.authorKong, M
dc.date.accessioned2025-01-21T01:31:56Z
dc.date.available2025-01-21T01:31:56Z
dc.date.issued1999
dc.description.abstractPrevious work from our laboratory indicated that gonadotrophin-releasing hormone (GnRH) increases human sperm-zona pellucida binding. Here we present evidence that GnRH antagonists inhibit sperm-zona pellucida binding in humans. Motile spermatozoa (10(7) cells/ml) were incubated in modified Tyrode's medium at 37 degrees C, in 5% CO2 in air. After 4.5 h, aliquots of spermatozoa were treated with saline (control) or with different concentrations of GnRH antagonists (test), Each sperm aliquot was then tested in the hemizona binding assay. In this assay, the control aliquot was incubated with half a human zona pellucida (hemizona) and the test aliquot was incubated with the matching half. After 20 min, the hemizonae were withdrawn and the number of zona-bound spermatozoa counted using phase-contrast microscopy, In addition, the effect of GnRH antagonists upon the pattern of sperm movement, frequency of sperm-zona pellucida collisions, and percentage of living and acrosome-reacted spermatozoa was determined. The results indicated that treatment with GnRH antagonists decreased the number of zona-bound spermatozoa and did not change the pattern of sperm movement, frequency of sperm-zona collisions, and percentage of acrosome-reacted spermatozoa, We suggest that this action of GnRH antagonists may be due to an effect on zona receptors on the sperm plasma membrane.
dc.fuente.origenWOS
dc.identifier.eissn1460-2350
dc.identifier.issn0268-1161
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97171
dc.identifier.wosidWOS:000081959700031
dc.issue.numero8
dc.language.isoen
dc.pagina.final2074
dc.pagina.inicio2069
dc.revistaHuman reproduction
dc.rightsacceso restringido
dc.subjectGnRH antagonists
dc.subjecthuman zona pellucida
dc.subjectsperm-zona collisions
dc.subjectsperm-zona binding
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleGonadotrophin-releasing hormone antagonists inhibit sperm binding to the human zona pellucida
dc.typeartículo
dc.volumen14
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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