Photochemical reactivity of the homologous proteinsα-lactalbumin and lysozyme

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dc.contributor.authorEdwards M., Ana M.
dc.contributor.authorSilva S., E.
dc.date.accessioned2019-12-28T02:45:32Z
dc.date.available2019-12-28T02:45:32Z
dc.date.issued1985
dc.description.abstractThe fluorescent behaviour and the photodynamic effect was studied in native and structurally modified lysozyme and?-lactalbumin.The Tyr residues in lysozyme and?-lactalbumin show different sensitivities to the photodynamic effect. The effect is zero in the case of Tyr from native lysozyme. In contrast, the Tyr residues in?-lactalbumin are susceptible to photooxidation, which indicates a greater degree of exposure to the solvent. The three His residues of?-lactalbumin have different degrees of exposure and show two different kinetics of photooxidation whereas the His residue of lysozyme is photooxidized with a single kinetic.Two photooxidation kinetics were obtained for the Trp residues of both native proteins, an indication that in both cases there are Trp residues that are differently exposed to the solvent. The wavelengths of maximum fluorescent emission of the Trp residues were different for the two proteins, an effect which can also be explained in terms of a difference in the environment of these residues. The modified form of these proteins emit at wavelengths longer than those of the native forms. When modified the proteins photooxidize with noticeably greater quantum yields.
dc.fuente.origenSpringer
dc.identifier.doi10.1007/BF01229820
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/27142
dc.identifier.urihttp://link.springer.com/article/10.1007/BF01229820
dc.issue.numeroNo. 2
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final148
dc.pagina.inicio141
dc.revistaRadiation and Environmental Biophysicses_ES
dc.rightsacceso restringido
dc.subject.ddc510
dc.subject.deweyMatemática física y químicaes_ES
dc.subject.otherFotoquímicaes_ES
dc.subject.otherLisozimaes_ES
dc.subject.otherCinéticaes_ES
dc.subject.otherBioquímica.es_ES
dc.titlePhotochemical reactivity of the homologous proteinsα-lactalbumin and lysozymees_ES
dc.typeartículo
dc.volumenVol. 24
sipa.codpersvinculados53657
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