Sulfated Polysaccharide Extracted from the Green Algae <i>Codium bernabei</i>: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity

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dc.contributor.authorFigueroa, Fabian A.
dc.contributor.authorAbdala-Diaz, Roberto T.
dc.contributor.authorPerez, Claudia
dc.contributor.authorCasas-Arrojo, Virginia
dc.contributor.authorNesic, Aleksandra
dc.contributor.authorTapia, Cecilia
dc.contributor.authorDuran, Carla
dc.contributor.authorValdes, Oscar
dc.contributor.authorParra, Carolina
dc.contributor.authorBravo-Arrepol, Gaston
dc.contributor.authorSoto, Luis
dc.contributor.authorBecerra, Jose
dc.contributor.authorCabrera-Barjas, Gustavo
dc.date.accessioned2025-01-20T21:03:59Z
dc.date.available2025-01-20T21:03:59Z
dc.date.issued2022
dc.description.abstractCodium bernabei is a green alga that grows on Chilean coasts. The composition of its structural polysaccharides is still unknown. Hence, the aim of this work is to isolate and characterize the hot water extracted polysaccharide fractions. For this purpose, the water extracts were further precipitated in alcohol (TPs) and acid media (APs), respectively. Both fractions were characterized using different physicochemical techniques such as GC-MS, GPC, FTIR, TGA, and SEM. It is confirmed that the extracted fractions are mainly made of sulfated galactan unit, with a degree of sulfation of 19.3% (TPs) and 17.4% (ATs) and a protein content of 3.5% in APs and 15.6% in TPs. Other neutral sugars such as xylose, glucose, galactose, fucose, mannose, and arabinose were found in a molar ratio (0.05:0.6:1.0:0.02:0.14:0.11) for TPs and (0.05:0.31:1.0:0.03:0.1:0.13) for ATs. The molecular weight of the polysaccharide samples was lower than 20 kDa. Both polysaccharides were thermally stable (Tonset > 190 degrees C) and showed antioxidant activity according to the ABTS(center dot+) and DPPH tests, where TPs fractions had higher scavenging activity (35%) compared to the APs fractions. The PT and APTTS assays were used to measure the anticoagulant activity of the polysaccharide fractions. In general, the PT activity of the TPs and APs was not different from normal plasma values. The exception was the TPs treatment at 1000 mu g mL(-1) concentration. The APTTS test revealed that clotting time for both polysaccharides was prolonged regarding normal values at 1000 mu g mL(-1). Finally, the antitumor test in colorectal carcinoma (HTC-116) cell line, breast cancer (MCF-7) and human leukemia (HL-60) cell lines showed the cytotoxic effect of TPs and APs. Those results suggest the potential biotechnological application of sulfate galactan polysaccharides isolated from a Chilean marine resource.
dc.fuente.origenWOS
dc.identifier.doi10.3390/md20070458
dc.identifier.eissn1660-3397
dc.identifier.urihttps://doi.org/10.3390/md20070458
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93200
dc.identifier.wosidWOS:000831939700001
dc.issue.numero7
dc.language.isoen
dc.revistaMarine drugs
dc.rightsacceso restringido
dc.subjectCodium bernabei
dc.subjectsulfated galactans
dc.subjectpolysaccharides
dc.subjectanticoagulant activity
dc.subjectantioxidant capacity
dc.subjectcytotoxic effect
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSulfated Polysaccharide Extracted from the Green Algae <i>Codium bernabei</i>: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity
dc.typeartículo
dc.volumen20
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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