Pex3p-Dependent Peroxisomal Biogenesis Initiates in the Endoplasmic Reticulum of Human Fibroblasts

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dc.contributor.authorToro, Andres A.
dc.contributor.authorAraya, Claudia A.
dc.contributor.authorCordova, Gonzalo J.
dc.contributor.authorArredondo, Cristian A.
dc.contributor.authorCardenas, Hugo G.
dc.contributor.authorMoreno, Regina E.
dc.contributor.authorVenegas, Alejandro
dc.contributor.authorKoenig, Cecilia S.
dc.contributor.authorCancino, Jorge
dc.contributor.authorGonzalez, Alfonso
dc.contributor.authorSantos, Manuel J.
dc.date.accessioned2024-01-10T12:07:30Z
dc.date.available2024-01-10T12:07:30Z
dc.date.issued2009
dc.description.abstractThe mechanisms of peroxisomal biogenesis remain incompletely understood, specially regarding the role of the endoplasmic reticulum (ER) in human cells, where genetic disorders of peroxisome biogenesis lead to Zellweger syndrome (ZS). The Pex3p peroxisomal membrane protein (PMP) required for early steps of peroxisome biogenesis has been detected in the ER in yeast but not in mammalian cells. Here, we show that Pex3p-GFP expressed in a new ZS cell line (MR), which lacks peroxisomes due to a mutation in the PEX3 gene, localizes first in the ER and subsequently in newly formed peroxisomes. Pex3p bearing an artificial N-glycosylation site shows an electrophoretic shift indicative of ER targeting while en route to preformed peroxisomes in normal fibroblast. A signal peptide that forces its entry into the ER does not eliminate its capability to drive peroxisome biogenesis in ZS cells. Thus, Pex3p is able to drive peroxisome biogenesis from the ER and its ER pathway is not privative of ZS cells. Cross-expression experiments of Pex3p in GM623 cells lacking Pex16p or Pex16p in MR cells lacking Pex3p, showed evidence that Pex3p requires Pex16p for ER location but: is dispensable for the ER location of Pex16p. These results indicate that Pex3p follows the ER-to-peroxisomal route in mammalian cells and provides new clues to understand its function. J. Cell. Biochem. 107: 10831096, 2009. (C) 2009 Wiley-Liss, Inc.
dc.description.funderFondo Nacional de Ciencia y Tecnologia (FONDECYT)
dc.description.funderFondo Nacional de Areas Prioritarias (FONDAP)
dc.description.funderVRAID
dc.fechaingreso.objetodigital2024-04-27
dc.format.extent14 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1002/jcb.22210
dc.identifier.eissn1097-4644
dc.identifier.issn0730-2312
dc.identifier.pubmedidMEDLINE:19479899
dc.identifier.urihttps://doi.org/10.1002/jcb.22210
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/76291
dc.identifier.wosidWOS:000268826900006
dc.information.autorucCiencias Biológicas;Araya CA;S/I;104964
dc.information.autorucCiencias Biológicas;Arredondo CA;S/I;11636
dc.information.autorucMedicina;González A;S/I;52306
dc.information.autorucCiencias Biológicas;Santos MJ;S/I;53987
dc.information.autorucCiencias Biológicas;Toro AA;S/I;5077
dc.issue.numero6
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final1096
dc.pagina.inicio1083
dc.publisherWILEY-BLACKWELL
dc.revistaJOURNAL OF CELLULAR BIOCHEMISTRY
dc.rightsacceso restringido
dc.subjectPEROXISOME
dc.subjectBIOGENESIS
dc.subjectENDOPLASMIC RETICULUM
dc.subjectCOMPLEMENTATION GROUP G
dc.subjectMEMBRANE-PROTEINS
dc.subjectZELLWEGER-SYNDROME
dc.subjectSACCHAROMYCES-CEREVISIAE
dc.subjectIMPORT RECEPTOR
dc.subjectRAT-LIVER
dc.subjectPEX3P
dc.subjectER
dc.subjectPATHWAY
dc.subjectPEX19
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePex3p-Dependent Peroxisomal Biogenesis Initiates in the Endoplasmic Reticulum of Human Fibroblasts
dc.typeartículo
dc.volumen107
sipa.codpersvinculados104964
sipa.codpersvinculados11636
sipa.codpersvinculados52306
sipa.codpersvinculados53987
sipa.codpersvinculados5077
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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