NPC2 is expressed in human and murine liver and secreted into bile: Potential implications for body cholesterol homeostasis

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dc.contributor.authorKlein, A
dc.contributor.authorAmigo, L
dc.contributor.authorRetamal, MJ
dc.contributor.authorMorales, MG
dc.contributor.authorMiquel, JF
dc.contributor.authorRigotti, A
dc.contributor.authorZanlungo, S
dc.date.accessioned2024-01-10T12:40:59Z
dc.date.available2024-01-10T12:40:59Z
dc.date.issued2006
dc.description.abstractThe liver plays a critical role in the metabolism of lipoprotein cholesterol and in controlling its elimination through the bile. Niemann-Pick type C 2 (NPC2), a cholesterol-binding protein, is key for normal intracellular trafficking of lipoprotein cholesterol, allowing its exit from the endolysosomal pathway into the metabolically active pool of the cell. In addition, NPC2 is a secretory protein from astrocytes and epididymal cells. Although NPC2 mRNA is detected in the liver, plasma and biliary NPC2 protein levels and function have not been reported. This study demonstrates that NPC2 is present in murine and human plasma and bile. In addition, hepatic NPC2 protein expression was dramatically increased in NPC1-deficient mice but not regulated by cholesterol feeding or pharmacological modulation of various nuclear receptors involved in cholesterol and bile acid metabolism. Interestingly, biliary NPC2 levels were 3-fold increased in gallstone-susceptible C57BL6/J versus gallstone-resistant BALB/c mice. Furthermore, NPC2 was exclusively found in the cholesterol pro-nucleating ConA-binding fraction of human bile. In conclusion, NPC2 is secreted from the liver into bile and plasma, where it may have a functional role in cholesterol transport in normal and disease conditions.
dc.fechaingreso.objetodigital05-04-2024
dc.format.extent8 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1002/hep.20985
dc.identifier.eissn1527-3350
dc.identifier.issn0270-9139
dc.identifier.pubmedidMEDLINE:16374838
dc.identifier.urihttps://doi.org/10.1002/hep.20985
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77368
dc.identifier.wosidWOS:000234460000018
dc.information.autorucMedicina;Miquel J;S/I;72216
dc.information.autorucMedicina;Rigotti A;S/I;68489
dc.information.autorucMedicina;Zanlungo S;S/I;72650
dc.issue.numero1
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final133
dc.pagina.inicio126
dc.publisherWILEY
dc.revistaHEPATOLOGY
dc.rightsacceso restringido
dc.subjectPICK-C DISEASE
dc.subjectHEPATIC CHOLESTEROL
dc.subjectGALLSTONE DISEASE
dc.subjectHUMAN EPIDIDYMIS
dc.subjectPROTEIN
dc.subjectMICE
dc.subjectCRYSTALLIZATION
dc.subjectMETABOLISM
dc.subjectDENSITY
dc.subjectTRANSPORT
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleNPC2 is expressed in human and murine liver and secreted into bile: Potential implications for body cholesterol homeostasis
dc.typeartículo
dc.volumen43
sipa.codpersvinculados72216
sipa.codpersvinculados68489
sipa.codpersvinculados72650
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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