Equilibrative nucleoside transporter 2 is expressed in human umbilical vein endothelium, but is not involved in the inhibition of adenosine transport induced by hyperglycaemia

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dc.contributor.authorAguayo, C
dc.contributor.authorCasado, J
dc.contributor.authorGonzalez, M
dc.contributor.authorPearson, JD
dc.contributor.authorSan Martin, R
dc.contributor.authorCasanello, P
dc.contributor.authorPastor Anglada, M
dc.contributor.authorSobrevia, L
dc.date.accessioned2024-01-10T13:48:07Z
dc.date.available2024-01-10T13:48:07Z
dc.date.issued2005
dc.description.abstractHuman equilibrative, Na+-independent nucleoside transport is mediated by membrane proteins sensitive (system es, hENT1) or insensitive (system ei, hENT2) to nitrobenzylthioinosine (NBMPR). Gestational diabetes and elevated extracellular concentrations of D-glucose reduce adenosine transport in human umbilical vein endothelium (HUVEC). We studied hENT2 and hENT1 expression in HUVEC, and the effect of D-glucose on their activity and expression in HUVEC preincubated with 25 MM D-glucose (24 h). hENT2 and hENT1 mRNA were quantified by real-time reverse transcription polymerase chain reaction, and their proteins were detected by Western blotting. hENT2 and hENT1 proteins are co-expressed in HUVEC and are located at the plasma membrane, however, hENT2 was mainly cytoplasmatic and perinuclear in location. D-Glucose reduced hENT1 and hENT2 mRNA expression, but only hENT1 protein abundance at the plasma membrane. Adenosine transport was inhibited by D-glucose and NMBPR (1 mu M) in intact cells and membrane vesicles. Hypoxanthine inhibited adenosine transport in the absence or in the presence of 1 mu M NBMPR. D-Glucose reduced NBMPR maximal binding in intact cells, membrane vesicles, and plasma membrane fractions. In conclusion, the present study demonstrates that hENT2 and hENT1 are co-expressed in HUVEC, and even when adenosine transport is also mediated by hENT2, the hENT2-mediated transport activity is not involved in the D-glucose-induced down-regulation of total adenosine transport.
dc.fechaingreso.objetodigital05-04-2024
dc.format.extent13 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.placenta.2004.10.006
dc.identifier.issn0143-4004
dc.identifier.pubmedidMEDLINE:16085043
dc.identifier.urihttps://doi.org/10.1016/j.placenta.2004.10.006
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79336
dc.identifier.wosidWOS:000231496500007
dc.information.autorucMedicina;Sobrevia L;S/I;1002656
dc.issue.numero8-9
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final653
dc.pagina.inicio641
dc.publisherW B SAUNDERS CO LTD
dc.revistaPLACENTA
dc.rightsacceso restringido
dc.subjectnucleoside
dc.subjecttransport
dc.subjectglucose
dc.subjectL-ARGININE TRANSPORT
dc.subjectPROTEIN-KINASE-C
dc.subjectNITRIC-OXIDE SYNTHESIS
dc.subjectD-GLUCOSE
dc.subjectDIFFERENT ISOFORMS
dc.subjectDIABETES-MELLITUS
dc.subjectHUMAN PLACENTA
dc.subjectSYSTEM Y(+)
dc.subjectCELLS
dc.subjectINSULIN
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleEquilibrative nucleoside transporter 2 is expressed in human umbilical vein endothelium, but is not involved in the inhibition of adenosine transport induced by hyperglycaemia
dc.typeartículo
dc.volumen26
sipa.codpersvinculados1002656
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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