Circulating platelet-derived microparticles in systemic lupus erythematosus - Association with increased thrombin generation and procoagulant state

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dc.contributor.authorPereira, J
dc.contributor.authorAlfaro, G
dc.contributor.authorGoycoolea, M
dc.contributor.authorQuiroga, T
dc.contributor.authorOcqueteau, M
dc.contributor.authorMassardo, L
dc.contributor.authorPerez, C
dc.contributor.authorSaez, C
dc.contributor.authorPanes, O
dc.contributor.authorMatus, V
dc.contributor.authorMezzano, D
dc.date.accessioned2024-01-10T12:38:02Z
dc.date.available2024-01-10T12:38:02Z
dc.date.issued2006
dc.description.abstractThe risk for thrombosis is significantly increased in systemic lupus erythematosus (SLE), affecting both venous and arterial vessels. Activated platelets are known to participate in thrombus formation and growth. A general feature of activated cells is the shedding of microparticles (MP) which support coagulation by exposure of negatively charged phospholipids and possibly tissue factor (TF). In this work we characterized circulating MP in patients with SLE and their relationship with a procoagulant state. Thirty patients with SLE (aged 15-72 years, mean age 38 years) and 20 healthy controls (aged 22-54 years, mean age 34 years) were studied; patients fulfilled 4 revised criteria for SLE. The number and cellular source of circulating MP were determined by flow cytometry using double labeling with specific monoclonal antibodies and annexin V. Thrombin generation was measured as the endogenous thrombin potential (ETP) without the addition of exogenous phospholipids and TF; under these conditions the generation of thrombin depended directly on the number of MP present in plasma. Thrombin anti-thrombin (TAT) and plasmin-antiplasmin (PAP) complexes were measured by ELISA. Compared to the controls, circulating MP were significantly elevated in the patient group (1218 +/- 136 vs 653 +/- 74 x 10(3)/ml plasma, p: 0.0007). In both groups, most of these MP were of platelet origin (927 +/- 131 vs 517 +/- 72 x 10(3)/ml plasma, p:0.009). ETP was higher among patients as compared to the controls (804 +/- 64 vs 631 +/- 37 nM thrombin, p: 0.025). Plasma levels of TAT in patients and controls were 3.4 +/- 0.8 and 1.4 +/- 0.5 mu g/L, respectively (p:0.04), and of PAP complexes were 62.5 +/- 14 and 24.05 +/- 2.5 mu g/ml, respectively (p:0.014). The number of platelet-derived MP correlated significantly with thrombin generation (r: 0.42; p: 0.038) and TAT levels (r: 0.40; p: 0.035). We did not find an association of circulating MP with disease activity nor with the presence of antiphospholipid antibodies. The increased number of circulating platelet-derived microparticles and their association with high ETP and activation of the coagulation system suggest that these microparticles play an important role in the pathogenesis of the prothrombotic state in SLE patients.
dc.fechaingreso.objetodigital2024-05-16
dc.format.extent6 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1160/TH05-05-0310
dc.identifier.eissn2567-689X
dc.identifier.issn0340-6245
dc.identifier.pubmedidMEDLINE:16543967
dc.identifier.urihttps://doi.org/10.1160/TH05-05-0310
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/76971
dc.identifier.wosidWOS:000234693100015
dc.information.autorucMedicina;Massardo L;S/I;100068
dc.information.autorucMedicina;Matus V;S/I;1744
dc.information.autorucMedicina;Mezzano D;S/I;99455
dc.information.autorucMedicina;Ocqueteau M;S/I;71377
dc.information.autorucMedicina;Panes O;S/I;90271
dc.information.autorucMedicina;Pereira J;S/I;99371
dc.information.autorucMedicina;Quiroga T;S/I;52601
dc.issue.numero1
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final99
dc.pagina.inicio94
dc.publisherGEORG THIEME VERLAG KG
dc.revistaTHROMBOSIS AND HAEMOSTASIS
dc.rightsacceso restringido
dc.subjectsystemic lupus erythematosus
dc.subjectmicroparticles
dc.subjectthrombin generation
dc.subjectACCELERATED ATHEROSCLEROSIS
dc.subjectENDOTHELIAL MICROPARTICLES
dc.subjectDISEASE-ACTIVITY
dc.subjectPLASMA-MEMBRANE
dc.subjectELEVATED LEVELS
dc.subjectCD40 LIGAND
dc.subjectIN-VITRO
dc.subjectAPOPTOSIS
dc.subjectACTIVATION
dc.subjectMECHANISM
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCirculating platelet-derived microparticles in systemic lupus erythematosus - Association with increased thrombin generation and procoagulant state
dc.typeartículo
dc.volumen95
sipa.codpersvinculados100068
sipa.codpersvinculados1744
sipa.codpersvinculados99455
sipa.codpersvinculados71377
sipa.codpersvinculados90271
sipa.codpersvinculados99371
sipa.codpersvinculados52601
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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