Inflammatory conditions induce gap junctional communication between rat Kupffer cells both <i>in vivo</i> and <i>in vitro</i>

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dc.contributor.authorEugenin, Eliseo A.
dc.contributor.authorGonzalez, Hernan E.
dc.contributor.authorSanchez, Helmuth A.
dc.contributor.authorBranes, Maria C.
dc.contributor.authorSaez, Juan C.
dc.date.accessioned2025-01-21T01:05:30Z
dc.date.available2025-01-21T01:05:30Z
dc.date.issued2007
dc.description.abstractConnexin43 (Cx43), a gap junction protein subunit, has been previously detected in Kupffer cells (KCs) during liver inflammation, however, KCs phagocytose cell debris that may include Cx43 protein, which could explain the detection of Cx43 in KCs. We determined that KCs express Cx43 and form gap junctions (GJs) both in vivo and in vitro. In liver sections of animals treated with LPS, Cx43 was detected at ED2+ cells interfaces, indicating formation of GJs between KCs in vivo. In vitro, unstimulated KCs cultures did not form functional GJs, and expressed low levels of Cx43 that showed a diffuse intracellular distribution. In contrast, KCs treated with LPS plus IFN-gamma, expressed a greater amount of Cx43 at both, protein and mRNA levels, and showed Cx43 at cell-cell contacts associated with higher dye coupling. In conclusion, activation of KCs in vivo or in vitro resulted in enhanced Cx43 expression levels and formation of GJ that might play relevant roles during liver inflammation. (c) 2007 Elsevier Inc. All rights reserved.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.cellimm.2007.08.001
dc.identifier.issn0008-8749
dc.identifier.urihttps://doi.org/10.1016/j.cellimm.2007.08.001
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95941
dc.identifier.wosidWOS:000250972200005
dc.issue.numero2
dc.language.isoen
dc.pagina.final110
dc.pagina.inicio103
dc.revistaCellular immunology
dc.rightsacceso restringido
dc.subjectconnexins
dc.subjectliver
dc.subjectdisease
dc.subjectmacrophages
dc.subjectinflammation
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleInflammatory conditions induce gap junctional communication between rat Kupffer cells both <i>in vivo</i> and <i>in vitro</i>
dc.typeartículo
dc.volumen247
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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