Role of natural transformation in the evolution of small cryptic plasmids in Synechocystis sp. PCC 6803

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dc.catalogadorjlo
dc.contributor.authorNies, Fabián
dc.contributor.authorWein, Tanita
dc.contributor.authorHanke, Dustin M.
dc.contributor.authorSpringstein, Benjamin L.
dc.contributor.authorAlcorta Loyola, Jaime Andrés
dc.contributor.authorTaubenheim, Claudia
dc.contributor.authorDagan, Tal
dc.date.accessioned2023-10-12T15:46:22Z
dc.date.available2023-10-12T15:46:22Z
dc.date.issued2023
dc.description.abstractSmall cryptic plasmids have no clear effect on the host fitness and theirfunctional repertoire remains obscure. The naturally competent cyanobacte-riumSynechocystissp. PCC 6803 harbours several small cryptic plasmids;whether their evolution with this species is supported by horizontal transferremains understudied. Here, we show that the small cryptic plasmid DNA istransferred in the population exclusively by natural transformation, wherethe transfer frequency of plasmid-encoded genes is similar to that ofchromosome-encoded genes. Establishing a system to follow gene transfer,we compared the transfer frequency of genes encoded in cryptic plasmidspCA2.4 (2378 bp) and pCB2.4 (2345 bp) within and between populations oftwoSynechocystissp. PCC 6803 labtypes (termed Kiel and Sevilla). Ourresults reveal that plasmid gene transfer frequency depends on the recipientlabtype. Furthermore, gene transfer via whole plasmid uptake in the Sevillalabtype ranged among the lowest detected transfer rates in our experi-ments. Our study indicates that horizontal DNA transfer via natural transfor-mation is frequent in the evolution of small cryptic plasmids that reside innaturally competent organisms. Furthermore, we suggest that the contribu-tion of natural transformation to cryptic plasmid persistence inSynechocys-tisis limited.
dc.fechaingreso.objetodigital2023-10-12
dc.format.extent13 páginas
dc.fuente.origenSIPA
dc.identifier.doi10.1111/1758-2229.13203
dc.identifier.issn1758-2229
dc.identifier.urihttps://doi.org/10.1111/1758-2229.13203
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75103
dc.information.autorucFacultad de Ciencias Biológicas; Alcorta Loyola, Jaime Andrés; 0000-0001-7662-239X; 187040
dc.issue.numero5
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final13
dc.pagina.inicio1
dc.revistaEnvironmental Microbiology Reports
dc.rightsacceso abierto
dc.subjectBiología
dc.subjectBacterias
dc.subjectMicrobiología
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.subject.ods06 Clean water and sanitation
dc.subject.odspa06 Agua limpia y saneamiento
dc.titleRole of natural transformation in the evolution of small cryptic plasmids in Synechocystis sp. PCC 6803
dc.typeartículo
dc.volumen15
sipa.codpersvinculados187040
sipa.trazabilidadORCID;2023-10-09
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