Differences in acute lung response to elastase instillation in two rodent species may determine differences in severity of emphysema development

Simple item page
dc.contributor.authorVecchiola, Andrea
dc.contributor.authorFrancisco de la Llera, Juan
dc.contributor.authorRamirez, Rodrigo
dc.contributor.authorOlmos, Pablo
dc.contributor.authorHerrera, Cristobal I.
dc.contributor.authorBorzone, Gisella
dc.date.accessioned2024-01-10T13:47:33Z
dc.date.available2024-01-10T13:47:33Z
dc.date.issued2011
dc.description.abstractVecchiola A, de la Llera JF, Ramirez R, Olmos P, Herrera CI, Borzone G. Differences in acute lung response to elastase instillation in two rodent species may determine differences in severity of emphysema development. Am J Physiol Regul Integr Comp Physiol 301: R148-R158, 2011. First published April 13, 2011; doi:10.1152/ajpregu.00133.2011.-Elastase intratracheal instillation induces early emphysema in rodents. However, Syrian Golden hamsters develop more severe emphysema than Sprague-Dawley rats. We have reported species differences in oxidant/antioxidant balance modulating antiprotease function early after instillation. We now hypothesize that other components of the initial lung response to elastase might also be species-dependent. Sprague-Dawley rats and Syrian Golden hamsters received a single dose of pancreatic elastase (0.55 U/100 g body wt) to study acute lung injury biomarkers. Using serum, lung, and bronchoalveolar lavage fluid (BALF) samples, we evaluated changes in alveolar-capillary permeability, alpha 1-antitrypsin (alpha(1)-AT) concentration and activity, glutathione content, and proinflammatory cytokines. Rats showed a large increase in alveolar-capillary permeability and few hemorrhagic changes, whereas hamsters exhibited large hemorrhagic changes (P < 0.01) and mild transendothelial passage of proteins. Western blots showed a 30-fold increase in BALF alpha(1)-AT concentration in rats and only a 7-fold increase in hamsters (P < 0.001), with [alpha(1)-AT-elastase] complexes only in rats, suggesting differences in antiprotease function. This was confirmed by the alpha(1)-AT bioassay showing 20-fold increase in alpha(1)-AT activity in rats and only twofold increase in hamsters (P < 0.001). In rats, results were preceded by a 3-, 60-, and 20-fold increase in IL-6, IL-1 beta, and TNF-alpha respectively (P < 0.001). In hamsters, only IL-1 beta and TNF-alpha showed mild increases. All parameters studied were back to baseline by 4 days. In conclusion, several components of the initial lung response showed species differences. Cytokine release pattern and functional inhibition of alpha(1)-AT were the most significant components differing among species and could account for differences in susceptibility to elastase.
dc.description.funderFONDECYT
dc.fechaingreso.objetodigital2024-05-14
dc.format.extent11 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1152/ajpregu.00133.2011
dc.identifier.eissn1522-1490
dc.identifier.issn0363-6119
dc.identifier.pubmedidMEDLINE:21490362
dc.identifier.urihttps://doi.org/10.1152/ajpregu.00133.2011
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79283
dc.identifier.wosidWOS:000292319800014
dc.information.autorucMedicina;Borzone G ;S/I;99892
dc.information.autorucMedicina;De la Llera J;S/I;169903
dc.information.autorucCiencias Biológicas;Herrera C ;S/I;156064
dc.information.autorucMedicina;Olmos P;S/I;98949
dc.information.autorucMedicina;Vecchiola A ;S/I;123319
dc.issue.numero1
dc.language.isoen
dc.nota.accesocontenido parcial
dc.publisherAMER PHYSIOLOGICAL SOC
dc.revistaAMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
dc.rightsacceso restringido
dc.subjectalpha(1)-antitrypsin
dc.subjectelastase
dc.subjectanimal models
dc.subjectacute lung injury
dc.subjecthemorrhage
dc.subjectexperimental
dc.subjectACUTE-PHASE RESPONSE
dc.subjectRESPIRATORY-DISTRESS-SYNDROME
dc.subjectINDUCED PULMONARY-EMPHYSEMA
dc.subjectCIGARETTE-SMOKE
dc.subjectALPHA(1)-PROTEINASE INHIBITOR
dc.subjectNEUTROPHIL ELASTASE
dc.subjectEPITHELIAL-CELLS
dc.subjectDESTRUCTION
dc.subjectINJURY
dc.subjectALPHA
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDifferences in acute lung response to elastase instillation in two rodent species may determine differences in severity of emphysema development
dc.typeartículo
dc.volumen301
sipa.codpersvinculados99892
sipa.codpersvinculados169903
sipa.codpersvinculados156064
sipa.codpersvinculados98949
sipa.codpersvinculados123319
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Differences in acute lung response to elastase instillation in two rodent species may determine differences in severity of emphysema development.pdf
Size:
3.51 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Artículos de revistas