Vertical Immobilization Method for Time-Lapse Microscopy Analysis in Filamentous Cyanobacteria

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dc.contributor.authorOlivares, Jorge
dc.contributor.authorGonzalez, Annia
dc.contributor.authorAndrade, Derly
dc.contributor.authorVasquez, Monica
dc.date.accessioned2025-01-20T17:16:08Z
dc.date.available2025-01-20T17:16:08Z
dc.date.issued2023
dc.description.abstractA main event in bacterial cell division is the septation process, where the protein FtsZ is the key element. FtsZ polymerizes forming a ring-like structure (Z-ring) in the middle of the cell that serves as a scaffold for other division proteins. Super-resolution microscopy in bacterial models Escherichia coli and Bacillus subtilis showed that the Z-ring is discontinuous, while live cell imaging studies demonstrated that FtsZ moves along the ring by a mechanism known as treadmilling. To study the dynamics of FtsZ in vivo, a special cell placement in a vertical position is necessary for imaging the complete structure of the ring in the XY plane. In the case of FtsZ imaging in multicellular cyanobacteria, such as Anabaena sp. PCC7120, maintaining the filaments in a vertical position is challenging because of the size of the cells and the filaments' length. In this article, we describe a method that allows the vertical immobilization of Anabaena sp. PCC 7120 filaments using low melting point agarose and syringes, to record the Z-ring in a mutant that expresses a FtsZ-sfGFP fusion protein. This method is a rapid and inexpensive way to register protein dynamics at the division site using confocal microscopy.
dc.fuente.origenWOS
dc.identifier.doi10.3791/65612
dc.identifier.issn1940-087X
dc.identifier.urihttps://doi.org/10.3791/65612
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91331
dc.identifier.wosidWOS:001159858200034
dc.issue.numero199
dc.language.isoen
dc.revistaJove-journal of visualized experiments
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleVertical Immobilization Method for Time-Lapse Microscopy Analysis in Filamentous Cyanobacteria
dc.typeartículo
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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