Analysis of the early response to spinal cord injury identified a key role for mTORC1 signaling in the activation of neural stem progenitor cells

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dc.contributor.authorPenailillo, Johany
dc.contributor.authorPalacios, Miriam
dc.contributor.authorMounieres, Constanza
dc.contributor.authorMunoz, Rosana
dc.contributor.authorSlater, Paula G.
dc.contributor.authorDe Domenico, Elena
dc.contributor.authorPatrushev, Ilya
dc.contributor.authorGilchrist, Mike
dc.contributor.authorLarrain, Juan
dc.date.accessioned2025-01-20T22:06:14Z
dc.date.available2025-01-20T22:06:14Z
dc.date.issued2021
dc.description.abstractXenopus laevis are able to regenerate the spinal cord during larvae stages through the activation of neural stem progenitor cells (NSPCs). Here we use high-resolution expression profiling to characterize the early transcriptome changes induced after spinal cord injury, aiming to identify the signals that trigger NSPC proliferation. The analysis delineates a pathway that starts with a rapid and transitory activation of immediate early genes, followed by migration processes and immune response genes, the pervasive increase of NSPC-specific ribosome biogenesis factors, and genes involved in stem cell proliferation. Western blot and immunofluorescence analysis showed that mTORC1 is rapidly and transiently activated after SCI, and its pharmacological inhibition impairs spinal cord regeneration and proliferation of NSPC through the downregulation of genes involved in the G1/S transition of cell cycle, with a strong effect on PCNA. We propose that the mTOR signaling pathway is a key player in the activation of NPSCs during the early steps of spinal cord regeneration.
dc.fuente.origenWOS
dc.identifier.doi10.1038/s41536-021-00179-3
dc.identifier.eissn2057-3995
dc.identifier.urihttps://doi.org/10.1038/s41536-021-00179-3
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/94197
dc.identifier.wosidWOS:000710132100001
dc.issue.numero1
dc.language.isoen
dc.revistaNpj regenerative medicine
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleAnalysis of the early response to spinal cord injury identified a key role for mTORC1 signaling in the activation of neural stem progenitor cells
dc.typeartículo
dc.volumen6
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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