Diversity and toxicity of the planktonic diatom genus<i> Pseudo-nitzschia</i> from coastal and offshore waters of the Southeast Pacific, including Pseudo-nitzschia dampieri sp. nov.

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dc.contributor.authorvon Dassow, Peter
dc.contributor.authorMikhno, Marta
dc.contributor.authorPercopo, Isabella
dc.contributor.authorOrellana, Valentina Rubio
dc.contributor.authorAguilera, Victor
dc.contributor.authorAlvarez, Gonzalo
dc.contributor.authorAraya, Michael
dc.contributor.authorCornejo-Guzman, Sebastian
dc.contributor.authorLlona, Tomas
dc.contributor.authorMardones, Jorge I.
dc.contributor.authorNorambuena, Luis
dc.contributor.authorSalas-Rojas, Victoria
dc.contributor.authorKooistra, Wiebe H. C. F.
dc.contributor.authorMontresor, Marina
dc.contributor.authorSarno, Diana
dc.date.accessioned2025-01-20T17:21:47Z
dc.date.available2025-01-20T17:21:47Z
dc.date.issued2023
dc.description.abstractTo expand knowledge of Pseudo-nitzschia species in the Southeast Pacific, we isolated specimens from coastal waters of central Chile (36 degrees S-30 degrees S), the Gulf of Corcovado, and the oceanic Robinson Crusoe Island (700 km offshore) and grew them into monoclonal strains. A total of 123 Pseudo-nitzschia strains were identified to 11 species based on sequencing of the ITS region of the nuclear rDNA and on ultrastructural and morphometric analyses of the frustule in selected representatives of each clade: P. australis, P. bucculenta, P. cf. chiniana, P. cf. decipiens, P. fraudulenta, P. hasleana, P. multistriata, P. plurisecta, P. cf. sabit, the new species P. dampieri sp. nov., and one undescribed species. Partial 18S and 28S rDNA sequences, including the hypervariable V4 and D1-D3 regions used for barcoding, were gathered from representative strains of each species to facilitate future metabarcoding studies. Results showed different levels of genetic, and at times ultrastructural, diversity among the above-mentioned entities, suggesting morphological variants (P. bucculenta), rapidly radiating complexes with ill-defined species boundaries (P. cf. decipiens and P. cf. sabit), and the presence of new species (P. dampieri sp. nov., Pseudo-nitzschia sp. 1, and probably P. cf. chiniana). Domoic acid (DA) was detected in 18 out of 82 strains tested, including those of P. australis, P. plurisecta, and P. multistriata. Toxicity varied among species mostly corresponding to expectations from previous reports, with the prominent exception of P. fraudulenta; DA was not detected in any of its 10 strains tested. In conclusion, a high diversity of Pseudo-nitzschia exists in Chilean waters, particularly offshore.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.hal.2023.102520
dc.identifier.eissn1878-1470
dc.identifier.issn1568-9883
dc.identifier.urihttps://doi.org/10.1016/j.hal.2023.102520
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91466
dc.identifier.wosidWOS:001119712200001
dc.language.isoen
dc.revistaHarmful algae
dc.rightsacceso restringido
dc.subjectPseudo-nitzschia
dc.subjectHumboldt Current
dc.subjectHarmful algal bloom
dc.subjectRobinson Crusoe Island
dc.subjectPhylogeny
dc.subjectDomoic acid
dc.subject.ods14 Life Below Water
dc.subject.odspa14 Vida submarina
dc.titleDiversity and toxicity of the planktonic diatom genus<i> Pseudo-nitzschia</i> from coastal and offshore waters of the Southeast Pacific, including Pseudo-nitzschia dampieri sp. nov.
dc.typeartículo
dc.volumen130
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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