Polycystin-2 Is Required for Starvation- and Rapamycin-Induced Atrophy in Myotubes

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dc.contributor.authorKretschmar, Catalina
dc.contributor.authorPena-Oyarzun, Daniel
dc.contributor.authorHernando, Cecilia
dc.contributor.authorHernandez-Moya, Nadia
dc.contributor.authorMolina-Berrios, Alfredo
dc.contributor.authorPaz Hernandez-Caceres, Maria
dc.contributor.authorLavandero, Sergio
dc.contributor.authorBudini, Mauricio
dc.contributor.authorMorselli, Eugenia
dc.contributor.authorParra, Valentina
dc.contributor.authorTroncoso, Rodrigo
dc.contributor.authorCriollo, Alfredo
dc.date.accessioned2025-01-23T21:15:37Z
dc.date.available2025-01-23T21:15:37Z
dc.date.issued2019
dc.description.abstractMuscle atrophy involves a massive catabolism of intracellular components leading to a significant reduction in cellular and tissue volume. In this regard, autophagy, an intracellular mechanism that degrades proteins and organelles, has been implicated with muscle breakdown. Recently, it has shown that polycystin-2 (PC2), a membrane protein that belongs to the transient receptor potential (TRP) family, is required for the maintenance of cellular proteostasis, by regulating autophagy in several cell types. The role of PC2 in the control of atrophy and autophagy in skeletal muscle remains unknown. Here, we show that PC2 is required for the induction of atrophy in C2C12 myotubes caused by nutrient deprivation or rapamycin exposure. Consistently, overexpression of PC2 induces atrophy in C2C12 myotubes as indicated by decreasing of the myogenic proteins myogenin and caveolin-3. In addition, we show that inhibition of mTORC1, by starvation or rapamycin is inhibited in cells when PC2 is silenced. Importantly, even if PC2 regulates mTORC1, our results show that the regulation of atrophy by PC2 is independent of autophagy. This study provides novel evidence regarding the role of PC2 in skeletal muscle cell atrophy.
dc.fuente.origenWOS
dc.identifier.doi10.3389/fendo.2019.00280
dc.identifier.issn1664-2392
dc.identifier.urihttps://doi.org/10.3389/fendo.2019.00280
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101097
dc.identifier.wosidWOS:000467304400001
dc.language.isoen
dc.revistaFrontiers in endocrinology
dc.rightsacceso restringido
dc.subjectatrophy
dc.subjectpolycystin-2
dc.subjectmyotubes
dc.subjectmTOR
dc.subjectstarvation
dc.subjectrapamycin
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePolycystin-2 Is Required for Starvation- and Rapamycin-Induced Atrophy in Myotubes
dc.typeartículo
dc.volumen10
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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