Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor.

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dc.contributor.authorGarcía Cañete, Patricia
dc.contributor.authorBizama, Carolina
dc.contributor.authorRosa, Lorena.
dc.contributor.authorCerda Infante, Javier
dc.contributor.authorSánchez, Marianela.
dc.contributor.authorMontecinos Acuña, Viviana
dc.contributor.authorAlfaro, Francisca.
dc.contributor.authorLeiva-Acevedo, Claudia.
dc.contributor.authorRomero, Diego.
dc.contributor.authorKato Cardemil, Sumie Rode
dc.date.accessioned2020-04-21T16:43:12Z
dc.date.available2020-04-21T16:43:12Z
dc.date.issued2020
dc.date.updated2020-04-19T00:04:23Z
dc.description.abstractAbstract Background Gallbladder cancer (GBC) is the most common tumor of the biliary tract. The incidence of GBC shows a large geographic variability, being particularly frequent in Native American populations. In Chile, GBC represents the second cause of cancer-related death among women. We describe here the establishment of three novel cell lines derived from the ascitic fluid of a Chilean GBC patient, who presented 46% European, 36% Mapuche, 12% Aymara and 6% African ancestry. Results After immunocytochemical staining of the primary cell culture, we isolated and comprehensively characterized three independent clones (PUC-GBC1, PUC-GBC2 and PUC-GBC3) by short tandem repeat DNA profiling and RNA sequencing as well as karyotype, doubling time, chemosensitivity, in vitro migration capability and in vivo tumorigenicity assay. Primary culture cells showed high expression of CK7, CK19, CA 19-9, MUC1 and MUC16, and negative expression of mesothelial markers. The three isolated clones displayed an epithelial phenotype and an abnormal structure and number of chromosomes. RNA sequencing confirmed the increased expression of cytokeratin and mucin genes, and also of TP53 and ERBB2 with some differences among the three cells lines, and revealed a novel exonic mutation in NF1. The PUC-GBC3 clone was the most aggressive according to histopathological features and the tumorigenic capacity in NSG mice. Conclusions The first cell lines established from a Chilean GBC patient represent a new model for studying GBC in patients of Native American descent.
dc.identifier.citationBiological Research. 2020 Apr 15;53(1):13
dc.identifier.doi10.1186/s40659-020-00282-7
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/28691
dc.issue.numeroNo. 13
dc.language.isoen
dc.pagina.final17
dc.pagina.inicio1
dc.revistaBiological Researches_ES
dc.rightsacceso abierto
dc.rights.holderThe Author(s)
dc.subjectGallbladder canceres_ES
dc.subjectCancer cell lineses_ES
dc.subjectAsciteses_ES
dc.subjectNative American ancestryes_ES
dc.subjectGene expression proflees_ES
dc.subject.ddc616.994
dc.subject.deweyMedicina y saludes_ES
dc.titleFunctional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor.es_ES
dc.typeartículo
dc.volumenVol. 53
sipa.codpersvinculados73909
sipa.codpersvinculados237745
sipa.codpersvinculados1012469
sipa.codpersvinculados111594
sipa.codpersvinculados82640
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