SIRT1 Regulates Dendritic Development in Hippocampal Neurons

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dc.contributor.authorCodocedo, Juan F.
dc.contributor.authorAllard, Claudio
dc.contributor.authorGodoy, Juan A.
dc.contributor.authorVarela-Nallar, Lorena
dc.contributor.authorInestrosa, Nibaldo C.
dc.date.accessioned2025-01-20T23:56:39Z
dc.date.available2025-01-20T23:56:39Z
dc.date.issued2012
dc.description.abstractDendritic arborization is required for proper neuronal connectivity. SIRT1, a NAD+ dependent histone deacetylase, has been associated to ageing and longevity, which in neurons is linked to neuronal differentiation and neuroprotection. In the present study, the role of SIRT1 in dendritic development was evaluated in cultured hippocampal neurons which were transfected at 3 days in vitro with a construct coding for SIRT1 or for the dominant negative SIRT1H363Y, which lacks the catalytic activity. Neurons overexpressing SIRT1 showed an increased dendritic arborization, while neurons overexpressing SIRT1H363Y showed a reduction in dendritic arbor complexity. The effect of SIRT1 was mimicked by treatment with resveratrol, a well known activator of SIRT1, which has no effect in neurons overexpressing SIRT1H363Y indicating that the effect of resveratrol was specifically mediated by SIRT1. Moreover, hippocampal neurons overexpressing SIRT1 were resistant to dendritic dystrophy induced by A beta aggregates, an effect that was dependent on the deacetylase activity of SIRT1. Our findings indicate that SIRT1 plays a role in the development and maintenance of dendritic branching in hippocampal neurons, and suggest that these effects are mediated by the ROCK signaling pathway.
dc.fuente.origenWOS
dc.identifier.doi10.1371/journal.pone.0047073
dc.identifier.issn1932-6203
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0047073
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95168
dc.identifier.wosidWOS:000309580800071
dc.issue.numero10
dc.language.isoen
dc.revistaPlos one
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSIRT1 Regulates Dendritic Development in Hippocampal Neurons
dc.typeartículo
dc.volumen7
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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