MATE1 expression in the cochlea and its potential involvement in cisplatin cellular uptake and ototoxicity

Simple item page
dc.contributor.authorWaissbluth, Sofia
dc.contributor.authorMartinez, Agustin D.
dc.contributor.authorFigueroa-Cares, Cindel
dc.contributor.authorSanchez, Helmuth A.
dc.contributor.authorMaass, Juan C.
dc.date.accessioned2025-01-20T20:16:16Z
dc.date.available2025-01-20T20:16:16Z
dc.date.issued2023
dc.description.abstractBackgroundCisplatin appears to enter the cochlear cells through the organic cation transporter 2 (OCT2). There is recent evidence that multidrug and toxin extrusion protein 1 (MATE1) is involved in cisplatin-induced nephrotoxicity. Its presence and role in the ear are unknown.Aims/ObjectivesEvaluate the presence and localization of MATE1, and determine the localization of OCT2, in the cochlea. Evaluate cisplatin uptake with regard to MATE1 and OCT2 expression.Material and MethodsMurine cochlear explants and paraffin-embedded cochleae were evaluated with immunohistochemistry for OCT2 and MATE1. Explant cultures were also treated with Texas Red cisplatin to determine their cellular uptake.ResultsMATE1 is present in the cochlea. Most intense labeling of MATE1 and OCT2 was seen in the outer hair cells (OHCs) and pillar cells, respectively. Both transporters were observed in the spiral ganglion neurons and stria vascularis. Expression levels of OCT2 and MATE1 decreased following cisplatin exposure. Texas Red cisplatin staining was strong in OHCs and pillar cells.Conclusions and SignificanceTo the best of our knowledge, this is the first study demonstrating the presence and localization of MATE1 in the cochlea. OCT2 labeling was seen in pillar cells. Consistently, OHCs and pillar cells uptake Texas Red cisplatin.
dc.fuente.origenWOS
dc.identifier.doi10.1080/00016489.2023.2184864
dc.identifier.eissn1651-2251
dc.identifier.issn0001-6489
dc.identifier.urihttps://doi.org/10.1080/00016489.2023.2184864
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/92318
dc.identifier.wosidWOS:000954093500001
dc.issue.numero3
dc.language.isoen
dc.pagina.final249
dc.pagina.inicio242
dc.revistaActa oto-laryngologica
dc.rightsacceso restringido
dc.subjectOCT2
dc.subjectMATE
dc.subjectMATE1
dc.subjectcisplatin
dc.subjectplatinum
dc.subjectototoxicity
dc.subjecttransporters
dc.subjectorganic cation transporter
dc.subjectpillar cell
dc.subjectouter hair cell
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleMATE1 expression in the cochlea and its potential involvement in cisplatin cellular uptake and ototoxicity
dc.typeartículo
dc.volumen143
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas