Vibrational spectroscopy characterization and anticoagulant activity of a sulfated polysaccharide from sea cucumber Athyonidium chilensis

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dc.contributor.authorMatsuhiro, Betty
dc.contributor.authorOsorio Roman, Igor O.
dc.contributor.authorTorres, Rodrigo
dc.date.accessioned2024-01-10T14:22:25Z
dc.date.available2024-01-10T14:22:25Z
dc.date.issued2012
dc.description.abstractExtraction of Athyonidium chilensis body wall afforded a light brown powder in 4.2% yield which was fractionated by ion-exchange chromatography. The main fraction F1 contained glucuronic acid, galactosamine and fucose in a molar ratio of 1.0:1.1:1.1, and 32.9% of sulfate group. The second derivative FT-IR spectroscopy and Surface-enhanced Raman scattering (SERS) give more information than the normal IR and Raman spectroscopies. The broad band in the normal FT-IR spectrum of F1 at 850.6 cm(-1) assigned to sulfate group was resolved in the second derivative spectrum into three bands at 856.4 cm(-1), 837.4 cm(-1) and 818.6 cm(-1) assigned to sulfate group attached to axial C4 and equatorial C2 and C6, respectively, in glycosidic residues. The most important bands in SERS of F1, but not observed in Raman spectrum are bands at 1648 cm(-1) (nu C=O), 1377 cm(-1) (delta C-H) assigned to methyl group of N-acetyl group, at 1173 cm(-1) and at 956.2 cm(-1) (nu C-O-C) of glycosidic linkage: and bands at 925.5 cm(-1) and 890.4 cm(-1) (delta C-1-H) assigned to a and beta anomeric configurations of glycosidic residues, respectively. By NMR studies, glycosidic linkages and sulfation pattern of F1 was confirmed. Results obtained indicated that fraction F1 is chondroitin 4,6-disulfate substituted at position O-3 of glucuronic acid by partially 2,4-disulfated alpha-fucopyranosyl residues. Moreover, the anticogulant activity, measure in APTT of F1 showed at low concentration, a good correlation with heparin. (C) 2012 Elsevier Ltd. All rights reserved.
dc.description.funderDICYT (Universidad de Santiago de Chile)
dc.description.funderCONICYT (Chile)
dc.fechaingreso.objetodigital21-03-2024
dc.format.extent7 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.carbpol.2012.01.052
dc.identifier.eissn1879-1344
dc.identifier.issn0144-8617
dc.identifier.urihttps://doi.org/10.1016/j.carbpol.2012.01.052
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79930
dc.identifier.wosidWOS:000302196200021
dc.information.autorucQuímica;Osorio I;S/I;207713
dc.issue.numero3
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final965
dc.pagina.inicio959
dc.publisherELSEVIER SCI LTD
dc.revistaCARBOHYDRATE POLYMERS
dc.rightsacceso restringido
dc.subjectAthyonidium chilensis
dc.subjectVibrational spectroscopy
dc.subjectFucosylated chondrointin sulfates
dc.subjectFUCOSYLATED CHONDROITIN SULFATE
dc.subjectSURFACE-ENHANCED RAMAN
dc.subjectBODY-WALL
dc.subjectINFRARED-SPECTROSCOPY
dc.subjectBLOCK FRACTIONS
dc.subjectGLYCOSAMINOGLYCANS
dc.subjectSPECTRA
dc.subjectACIDS
dc.subjectIR
dc.subjectHEPARIN
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleVibrational spectroscopy characterization and anticoagulant activity of a sulfated polysaccharide from sea cucumber Athyonidium chilensis
dc.typeartículo
dc.volumen88
sipa.codpersvinculados207713
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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