Comparison of features of human breast cancer cell lines and their corresponding tumors

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dc.contributor.authorWistuba, II
dc.contributor.authorBehrens, C
dc.contributor.authorMilchgrub, S
dc.contributor.authorSyed, S
dc.contributor.authorAhmadian, M
dc.contributor.authorVirmani, AK
dc.contributor.authorKurvari, V
dc.contributor.authorCunningham, TH
dc.contributor.authorAshfaq, R
dc.contributor.authorMinna, JD
dc.contributor.authorGazdar, AF
dc.date.accessioned2024-01-10T13:11:55Z
dc.date.available2024-01-10T13:11:55Z
dc.date.issued1998
dc.description.abstractAlthough human tumor-derived cell lines play an important role in the investigation of cancer biology and genetics, there is no comprehensive study comparing tumor cell line properties with those of the individual tumors from which they were derived. We compared the properties of a series of 18 human breast cancer cell lines that were cultured for a median period of 25 months (range, 9-60 months) and their corresponding archival tumor tissues. We compared morphological characteristics, ploidy, and immunohistochemical expression of estrogen receptors, progesterone receptors, and HER2/neu and p53 proteins. For 17 of these cases, we also tested for allelic losses at 18 chromosomal regions frequently deleted in breast tumors using 51 polymorphic microsatellite markers, and we determined the TP53 gene mutation status in exons 5 to 10, There was an excellent correlation between the breast tumor cell lines and their corresponding tumor tissues for morphological features (100%); presence of aneuploidy (87%); immunohistochemical expression of estrogen receptors (87%), progesterone receptors (73%), and HER2/neu (93%) and p53 proteins (100%); allelic loss at all of the chromosomal regions analyzed (82-100% concordance); and TP53 gene mutations (75%), The same parental allele was lost in 279 (99%) of 281 of the comparisons of allele losses. The fractional allelic loss indices (a reflection of the total allelic loss) of the cell lines and their corresponding tumor tissues were identical or similar in 15 (88%) of 17 paired comparisons, Although our previous studies (A, Gazdar et at, Int. J, Cancer, in press) indicated that only a subset of primary breast carcinomas that have several features indicative of advanced tumors with poor prognosis can be successfully cultured, the cell lines retain the properties of their parental tumors for lengthy culture periods and, thus, provide suitable model systems for biomedical studies.
dc.format.extent8 páginas
dc.fuente.origenWOS
dc.identifier.eissn1557-3265
dc.identifier.issn1078-0432
dc.identifier.pubmedidMEDLINE:9865903
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78114
dc.identifier.wosidWOS:000077423700002
dc.information.autorucMedicina;Wistuba I;S/I;100278
dc.issue.numero12
dc.language.isoen
dc.nota.accesoSin adjunto
dc.pagina.final2938
dc.pagina.inicio2931
dc.publisherAMER ASSOC CANCER RESEARCH
dc.revistaCLINICAL CANCER RESEARCH
dc.rightsregistro bibliográfico
dc.subjectALLELE LOSS
dc.subjectGENETIC ALTERATIONS
dc.subjectPROGNOSTIC FACTORS
dc.subjectCHROMOSOME 3P
dc.subjectREGIONS
dc.subjectHETEROZYGOSITY
dc.subjectCARCINOMA
dc.subjectFREQUENT
dc.subjectOVEREXPRESSION
dc.subjectAMPLIFICATION
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleComparison of features of human breast cancer cell lines and their corresponding tumors
dc.typeartículo
dc.volumen4
sipa.codpersvinculados100278
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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