Spinal Cord Transection In <i>Xenopus laevis</i> Tadpoles

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dc.contributor.authorSlater, Paula G.
dc.contributor.authorLarrain, Juan
dc.date.accessioned2025-01-20T22:02:13Z
dc.date.available2025-01-20T22:02:13Z
dc.date.issued2021
dc.description.abstractSpinal cord injury (SCI) is a permanent affliction, which affects the central nervous system (CNS) motor and sensory nerves, resulting in paralysis beneath the injury site. To date, there is no functional recovery therapy for SCI, and there is a lack of clarity regarding the many complexes and dynamic events occurring after SCI. Many non-mammalian organisms can regenerate after severe SCI, such as teleost fishes, urodele amphibians, and larval stages of anuran amphibians, including Xenopus laevis tadpoles. These are bona fide model organisms to study and understand the response to SCI and the mechanisms underlying successful regenerative processes. This type of research can lead to the identification of potential targets for SCI therapeutic intervention. This article describes how to perform Xenopus laevis tadpole spinal cord transection, including husbandry, surgery, postsurgery care, and functional test evaluation. This injury method can be applied for elucidating the different steps of spinal cord regeneration by studying the cellular, molecular, and genetic mechanisms, as well as histological and functional evolution after SCI and during spinal cord regeneration.
dc.fuente.origenWOS
dc.identifier.doi10.3791/63276
dc.identifier.issn1940-087X
dc.identifier.urihttps://doi.org/10.3791/63276
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93952
dc.identifier.wosidWOS:000802600300058
dc.issue.numero178
dc.language.isoen
dc.revistaJove-journal of visualized experiments
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSpinal Cord Transection In <i>Xenopus laevis</i> Tadpoles
dc.typeartículo
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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