Collateral Sprouting of Peripheral Sensory Neurons Exhibits a Unique Transcriptomic Profile

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dc.contributor.authorLemaitre, Dominique
dc.contributor.authorLlavero Hurtado, Maica
dc.contributor.authorDe Gregorio, Cristian
dc.contributor.authorOnate, Maritza
dc.contributor.authorMartinez, Gabriela
dc.contributor.authorCatenaccio, Alejandra
dc.contributor.authorWishart, Thomas M.
dc.contributor.authorCourt, Felipe A.
dc.date.accessioned2025-01-23T19:50:15Z
dc.date.available2025-01-23T19:50:15Z
dc.date.issued2020
dc.description.abstractPeripheral nerve injuries result in motor and sensory dysfunction which can be recovered by compensatory or regenerative processes. In situations where axonal regeneration of injured neurons is hampered, compensation by collateral sprouting from uninjured neurons contributes to target reinnervation and functional recovery. Interestingly, this process of collateral sprouting from uninjured neurons has been associated with the activation of growth-associated programs triggered by Wallerian degeneration. Nevertheless, the molecular alterations at the transcriptomic level associated with these compensatory growth mechanisms remain to be fully elucidated. We generated a surgical model of partial sciatic nerve injury in mice to mechanistically study degeneration-induced collateral sprouting from spared fibers in the peripheral nervous system. Using next-generation sequencing and Ingenuity Pathway Analysis, we described the sprouting-associated transcriptome of uninjured sensory neurons and compare it with the activated by regenerating neurons. In vitro approaches were used to functionally assess sprouting gene candidates in the mechanisms of axonal growth. Using a novel animal model, we provide the first description of the sprouting transcriptome observed in uninjured sensory neurons after nerve injury. This collateral sprouting-associated transcriptome differs from that seen in regenerating neurons, suggesting a molecular program distinct from axonal growth. We further demonstrate that genetic upregulation of novel sprouting-associated genes activates a specific growth program in vitro, leading to increased neuronal branching. These results contribute to our understanding of the molecular mechanisms associated with collateral sprouting in vivo. The data provided here will therefore be instrumental in developing therapeutic strategies aimed at promoting functional recovery after injury to the nervous system.
dc.fuente.origenWOS
dc.identifier.doi10.1007/s12035-020-01986-3
dc.identifier.eissn1559-1182
dc.identifier.issn0893-7648
dc.identifier.urihttps://doi.org/10.1007/s12035-020-01986-3
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100544
dc.identifier.wosidWOS:000551056200001
dc.issue.numero10
dc.language.isoen
dc.pagina.final4249
dc.pagina.inicio4232
dc.revistaMolecular neurobiology
dc.rightsacceso restringido
dc.subjectAxonal regeneration
dc.subjectCollateral sprouting
dc.subjectNerve injury
dc.subjectSciatic nerve
dc.subjectTranscriptome
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCollateral Sprouting of Peripheral Sensory Neurons Exhibits a Unique Transcriptomic Profile
dc.typeartículo
dc.volumen57
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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