Organogenesis in vitro of Nothofagus alpina (P et E) Oerst, Fagaceae
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Date
1996
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Abstract
In vitro shoot and root regeneration of 2-year-old Nothofagus alpina plants was achieved from several types of explants cultured in vitro on a modified Woody Plant Medium formulation. Multiple shoot formation was obtained from leaf explants using 0.45-2.27 mu M thidiazuron and 0.0049-0.098 mu M indolebutyric acid. Excised axillary buds formed shoots and roots in the presence of 0.0049 mu M benzyladenine and 2.46 mu M indolebutyric acid, or in the absence of plant growth regulators. Nodal sections rooted when 2.46 mu M indole butyric acid at was supplied in the medium. Subcultured shoots originating from nodal sections showed a high regeneration rate through multiple shoot and root formation.