Rapid <i>in vitro</i> propagation and microtuber production in <i>Ullucus tuberosus</i> (Basellaceae)

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dc.contributor.authorJordan, M
dc.contributor.authorAmenábar, A
dc.contributor.authorRoveraro, C
dc.date.accessioned2025-01-21T01:30:29Z
dc.date.available2025-01-21T01:30:29Z
dc.date.issued2002
dc.description.abstractA rapid in vitro propagation system leading to the generation of plantlets and microtubers was developed for Ullucus tuberosus. The method consisted of a two phase culture. The first started with nodal sections, each showing 3-4 axillary buds, and was the source of a large number of new shoots (max. 18.3 shoots/ flask) which formed within 15 days. Development of new shoots by axillary-bud sprouting and profuse shoot branching took place after 2-4 days in the presence of MS-media supplemented with various growth regulators including NAA, GA(3), BA or Z (Zeatin). The second phase, taking place after 15 days, consisted in subculturing single shoots to promote the production of roots and microtubers. Single shoots, transferred to a liquid (chromatogram paper-bridges) or jellyfied MS-agar medium in various levels of the indicated plant growth regulators, developed roots forming plantlets with I or more microtubers within 2 months. The highest tuberization yield was obtained in the presence of NAA (0.3 mg 1(-1)), BA (0.1 mg 1(-1)) and GA3 (0.01 mg 1(-1)) resulting in approx. 83 % tuber formation.
dc.fuente.origenWOS
dc.identifier.issn0016-478X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96778
dc.identifier.wosidWOS:000176151100002
dc.issue.numero2
dc.language.isoen
dc.pagina.final54
dc.pagina.inicio50
dc.revistaGartenbauwissenschaft
dc.rightsacceso restringido
dc.subject.ods15 Life on Land
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.titleRapid <i>in vitro</i> propagation and microtuber production in <i>Ullucus tuberosus</i> (Basellaceae)
dc.typeartículo
dc.volumen67
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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