Phosphorylation of nuclear proteins directs binding to salicylic acid-responsive elements

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dc.contributor.authorStange, C
dc.contributor.authorRamirez, I
dc.contributor.authorGomez, I
dc.contributor.authorJordana, X
dc.contributor.authorHoluigue, L
dc.date.accessioned2025-01-21T01:33:17Z
dc.date.available2025-01-21T01:33:17Z
dc.date.issued1997
dc.description.abstractThe cis-located DNA sequence as-1 (Activation Sequence-1) from CaMV 35S promoter has been previously identified as an element that can confer inducibility by salicylic acid (SA) with immediate early kinetics. This sequence specifically binds to ASF-1 (Activation Sequence Factor-1), previously characterized in tobacco nuclear extracts. To assess whether modulation of ASF-1 binding activity can explain the activation of the as-1 sequence observed in vivo, we performed electrophoretic mobility shift assays using nuclear extracts from SA-treated and water-treated tobacco plants. Our results indicate that treatment of plants with SA increases ASF-1 binding to as-1 and to ocs, an as-1-like element from the Agrobacterium octopine synthase gene. In contrast, SA treatment has no effect on the binding of GT-1 factor to its target light-inducible box II element. Furthermore, treatment of nuclear extracts from SA-treated plants with alkaline phosphatase decreases ASF-1 binding to the as-1 element. This can be reversed by pretreatment with 10 mM NaF. Accordingly, pretreatment of nuclear extracts from control water-treated plants with ATP produces an increase in ASF-1 binding activity similar to that observed with SA. This effect of ATP is reversed by treatment with alkaline phosphatase and prevented by quercetin, a casein kinase II inhibitor. These results support the hypothesis that a nuclear protein kinase is involved in the immediate early events of transcriptional activation triggered by SA.
dc.fuente.origenWOS
dc.identifier.issn0960-7412
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97400
dc.identifier.wosidWOS:A1997XG77100016
dc.issue.numero6
dc.language.isoen
dc.pagina.final1324
dc.pagina.inicio1315
dc.revistaPlant journal
dc.rightsacceso restringido
dc.subject.ods13 Climate Action
dc.subject.ods02 Zero Hunger
dc.subject.odspa13 Acción por el clima
dc.subject.odspa02 Hambre cero
dc.titlePhosphorylation of nuclear proteins directs binding to salicylic acid-responsive elements
dc.typeartículo
dc.volumen11
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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