Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

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dc.article.number64604
dc.catalogadoraba
dc.contributor.authorFigueroa, A.-M.
dc.contributor.authorStolzenbach, F.
dc.contributor.authorTapia, P.
dc.contributor.authorCortés, V.
dc.date.accessioned2024-03-04T15:19:41Z
dc.date.available2024-03-04T15:19:41Z
dc.date.issued2023
dc.description.abstractBrown adipose tissue (BAT) is only present in mammals and has a thermogenic function. Brown adipocytes are characterized by a multilocular cytoplasm with multiple lipid droplets, a central nucleus, a high mitochondrial content, and the expression of uncoupling protein 1 (UCP1). BAT has been proposed as a potential therapeutic target for obesity and its associated metabolic disorders due to its ability to dissipate metabolic energy as heat. To investigate BAT function and regulation, brown adipocyte culturing is indispensable. The present protocol optimizes tissue processing and cell differentiation for culturing brown adipocytes from newborn mice. Additionally, procedures for the imaging of differentiated adipocytes with both confocal immunofluorescence and transmission electron microscopy are shown. In the brown adipocytes differentiated with the techniques described herein, the major defining features of classical BAT are preserved, including high UCP1 levels, increased mitochondrial mass, and very close physical contact between the lipid droplets and mitochondria, making this method a valuable tool for BAT studies.
dc.fechaingreso.objetodigital2024-09-11
dc.format.extent18 páginas
dc.fuente.origenORCID
dc.identifier.doi10.3791/64604
dc.identifier.eissn2578-126X
dc.identifier.urihttps://doi.org/10.3791/64604
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/82464
dc.identifier.wosidWOS:000946321000039
dc.information.autorucEscuela de Medicina; Cortes Mora, Victor Antonio; 0000-0002-1658-0965; 7576
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final18
dc.pagina.inicio1
dc.revistajove
dc.rightsacceso restringido
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDifferentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
dc.typeartículo
sipa.codpersvinculados7576
sipa.trazabilidadORCID;2024-01-15
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