PSD95 regulates morphological development of adult-born granule neurons in the mouse hippocampus

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dc.contributor.authorMardones, Muriel D.
dc.contributor.authorJorquera, Patricia, V
dc.contributor.authorHerrera-Soto, Andrea
dc.contributor.authorAmpuero, Estibaliz
dc.contributor.authorBustos, Fernando J.
dc.contributor.authorvan Zundert, Brigitte
dc.contributor.authorVarela-Nallar, Lorena
dc.date.accessioned2025-01-23T21:12:51Z
dc.date.available2025-01-23T21:12:51Z
dc.date.issued2019
dc.description.abstractIn the adult hippocampus new neurons are generated in the dentate gyrus from neural progenitor cells. Adult-born neurons integrate into the hippocampal circuitry and contribute to hippocampal function. PSD95 is a major postsynaptic scaffold protein that is crucial for morphological maturation and synaptic development of hippocampal neurons. Here we study the function of PSD95 in adult hippocampal neurogenesis by downregulating PSD95 expression in newborn cells using retroviral-mediated RNA interference. Retroviruses coding for a control shRNA or an shRNA targeting PSD95 (shPSD95) were stereotaxically injected into the dorsal dentate gyms of 2-month-old C57BL/6 mice. PSD95 knockdown did not affect neuronal differentiation of newborn cells into neurons, or migration of newborn neurons into the granule cell layer. Morphological analysis revealed that newborn neurons expressing shPSD95 showed increased dendritic length and increased number of high-order dendrites. Concomitantly, dendrites from shPSD95-expressing newborn granule neurons showed a reduction in the density of dendritic spines. These results suggest that PSD95 is required for proper dendritic and spine maturation of adult-born neurons, but not for early stages of neurogenesis in the hippocampus.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.jchemneu.2019.04.009
dc.identifier.eissn1873-6300
dc.identifier.issn0891-0618
dc.identifier.urihttps://doi.org/10.1016/j.jchemneu.2019.04.009
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100978
dc.identifier.wosidWOS:000470953800013
dc.language.isoen
dc.pagina.final123
dc.pagina.inicio117
dc.revistaJournal of chemical neuroanatomy
dc.rightsacceso restringido
dc.subjectPSD95
dc.subjectNeurogenesis
dc.subjectAdult
dc.subjectHippocampus
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePSD95 regulates morphological development of adult-born granule neurons in the mouse hippocampus
dc.typeartículo
dc.volumen98
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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