Stable Transformation of <i>Arabidopsis thaliana</i> Cell Suspension Cultures: A Case Study for The Overexpression of the COI1 Receptor

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dc.contributor.authorDiaz-Sanchez, Eva K.
dc.contributor.authorO'Brien, Jose A.
dc.contributor.authorPerez-Salamo, Imma
dc.contributor.authorKrasauskas, Jovaras
dc.contributor.authorBomer, Moritz
dc.contributor.authorDevoto, Alessandra
dc.date.accessioned2025-01-20T23:54:47Z
dc.date.available2025-01-20T23:54:47Z
dc.date.issued2021
dc.description.abstractCell suspension cultures have been studied for decades to produce natural molecules. However, the difficulty in generating stably transformed cell lines has limited their use to produce high value chemicals reproducibly and in elevated quantities.
dc.description.abstractIn this protocol, a method to stably transform and maintain Arabidopsis cell suspension cultures is devised and presented in detail. Arabidopsis cell cultures were directly transformed with A. tumefaciens for the overexpression of the CORONATINE INSENSITIVE 1 (COI1) jasmonate receptor. Cell cultures were established after transformation and continuously maintained and tested for the overexpression of COI1. The protocol was also previously used to silence Arabidopsis peroxidases and allows for long term maintenance of transformed cells. Details on culture maintenance, both in liquid and solid media are provided, alongside with evidence of protein expression to confirm transformation.
dc.description.abstractThe system described provides a powerful tool for synthetic biology to study signaling independent of developmental control and to obtain metabolites of interest for the biotechnological and medical sectors.
dc.fuente.origenWOS
dc.identifier.doi10.21769/BioProtoc.3880
dc.identifier.eissn2331-8325
dc.identifier.urihttps://doi.org/10.21769/BioProtoc.3880
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95050
dc.identifier.wosidWOS:000606442100013
dc.issue.numero1
dc.language.isoen
dc.revistaBio-protocol
dc.rightsacceso restringido
dc.subjectArabidopsis
dc.subjectAgrobacterium tumefaciens
dc.subjectStable plant cell suspension culture
dc.subjectTransformation
dc.subjectMetabolites
dc.subject.ods15 Life on Land
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.titleStable Transformation of <i>Arabidopsis thaliana</i> Cell Suspension Cultures: A Case Study for The Overexpression of the COI1 Receptor
dc.typeartículo
dc.volumen11
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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