Cellular immune responses in amniotic fluid of women with preterm clinical chorioamnionitis

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dc.contributor.authorGalaz, Jose
dc.contributor.authorRomero, Roberto
dc.contributor.authorXu, Yi
dc.contributor.authorMiller, Derek
dc.contributor.authorSlutsky, Rebecca
dc.contributor.authorLevenson, Dustyn
dc.contributor.authorHsu, Chaur-Dong
dc.contributor.authorGomez-Lopez, Nardhy
dc.date.accessioned2025-01-23T19:54:36Z
dc.date.available2025-01-23T19:54:36Z
dc.date.issued2020
dc.description.abstractObjective Preterm birth is the leading cause of neonatal morbidity and mortality worldwide. Some preterm births are associated with clinical chorioamnionitis; yet, this condition has been poorly investigated. Herein, we characterized the amniotic fluid cellular immune responses in women with preterm clinical chorioamnionitis. Methods and subjects Amniotic fluid samples were obtained from women with preterm clinical chorioamnionitis and a positive or negative microbiological culture (n = 17). The cellular composition of amniotic fluid was evaluated using fluorescence microscopy, scanning and transmission electron microscopy, and flow cytometry. Women without preterm clinical chorioamnionitis were also examined (n = 10). Results Amniotic fluid from women with preterm clinical chorioamnionitis and a positive culture had: (1) abundant neutrophils associated with viable and non-viable bacteria, (2) neutrophils performing phagocytosis, (3) neutrophils forming NETs, (4) increased numbers of neutrophils, monocytes/macrophages, and CD4+ T cells, and (5) high expression of IL-1 beta by neutrophils and monocytes/macrophages. Amniotic fluid from women with preterm clinical chorioamnionitis and proven infection tended to have fewer monocytes/macrophages and CD4+ T cells compared to those without chorioamnionitis. Conclusion We provide the first morphologic and phenotypic characterization of the cellular immune responses in the amniotic cavity of women with preterm clinical chorioamnionitis, a condition associated with adverse neonatal outcomes.
dc.fuente.origenWOS
dc.identifier.doi10.1007/s00011-019-01308-x
dc.identifier.eissn1420-908X
dc.identifier.issn1023-3830
dc.identifier.urihttps://doi.org/10.1007/s00011-019-01308-x
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100691
dc.identifier.wosidWOS:000511956900007
dc.issue.numero2
dc.language.isoen
dc.pagina.final216
dc.pagina.inicio203
dc.revistaInflammation research
dc.rightsacceso restringido
dc.subjectAcute chorioamnionitis
dc.subjectImmune Cells
dc.subjectImmunology
dc.subjectNeutrophils
dc.subjectMonocytes
dc.subjectMacrophages
dc.subjectT Cells
dc.subject.ods05 Gender Equality
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa05 Igualdad de género
dc.subject.odspa03 Salud y bienestar
dc.titleCellular immune responses in amniotic fluid of women with preterm clinical chorioamnionitis
dc.typeartículo
dc.volumen69
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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