Fatty acid composition of <i>Drosophila</i> photoreceptor light-sensitive microvilli

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dc.contributor.authorMunoz, Yorka
dc.contributor.authorFuenzalida, Karen
dc.contributor.authorBronfman, Miguel
dc.contributor.authorGatica, Arnaldo
dc.contributor.authorSepulveda, Marcelo
dc.contributor.authorBacigalupo, Juan
dc.contributor.authorRoth, Alejandro D.
dc.contributor.authorDelgado, Ricardo
dc.date.accessioned2025-01-24T00:07:58Z
dc.date.available2025-01-24T00:07:58Z
dc.date.issued2013
dc.description.abstractPhototransduction, the mechanism underlying the electrical response to light in photoreceptor cells, has been thoroughly investigated in Drosophila melanogaster, an essential model in signal transduction research. These cells present a highly specialized photosensitive membrane consisting of thousands of microvilli forming a prominent structure termed a rhabdomere. These microvilli encompass the phototransduction proteins, most of which are transmembrane and exclusively rhabdomeric. Rhabdomere membrane lipids play a crucial role in the activation of the transient receptor potential ionic channels (TRP and TRPL) responsible for initiating the photoresponse. Despite its importance, rhabdomere lipid composition has not been established. We developed a novel preparation enriched in rhabdomere membranes to perform a thorough characterization of the lipidomics of Drosophila rhabdomeres. Isolated eyes (500) were homogenized and subjected to a differential centrifugation protocol that generates a fraction enriched in rhabdomere membrane. Lipids extracted from this preparation were identified and quantified by gas chromatography coupled to mass spectrometry. We found an abundance of low sterol esters (C16:0, C18:0), highly abundant and diverse triglycerides, free fatty acids, a moderate variety of mono and diacyglycerols (C:16:0, 18:0, C18:1) and abundant phospholipids (principally C18:2). This preparation opens a new avenue for investigating essential aspects of phototransduction.
dc.fuente.origenWOS
dc.identifier.eissn0717-6287
dc.identifier.issn0716-9760
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101937
dc.identifier.wosidWOS:000326205100010
dc.issue.numero3
dc.language.isoen
dc.pagina.final294
dc.pagina.inicio289
dc.revistaBiological research
dc.rightsacceso restringido
dc.subjectRhabdomere
dc.subjectDrosophila
dc.subjectmicrovilli
dc.subjectphototransduction
dc.subjectTRP channel
dc.subjectfatty acid analysis
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleFatty acid composition of <i>Drosophila</i> photoreceptor light-sensitive microvilli
dc.typeartículo
dc.volumen46
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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