Differential expression of canalicular membrane Ca2+/Mg2+-ecto-ATPase in estrogen-induced and obstructive cholestasis in the rat

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dc.contributor.authorAccatino, L
dc.contributor.authorPizarro, M
dc.contributor.authorSolis, N
dc.contributor.authorArrese, M
dc.contributor.authorVollrath, V
dc.contributor.authorAnanthanarayanan, M
dc.contributor.authorChianale, J
dc.contributor.authorKoenig, CS
dc.date.accessioned2024-01-10T12:38:22Z
dc.date.available2024-01-10T12:38:22Z
dc.date.issued2000
dc.description.abstractExtracellular adenosine triphosphate (ATP) may regulate hepatocyte and cholangiocyte functions, and under some conditions it may have deleterious effects on bile secretion and cause cholestasis. The canalicular membrane enzyme Ca2+/Mg2+-ecto-ATPase (ecto-ATPase) hydrolyzes ATP/adenosine diphosphate (ATP/ADP) and regulates hepatic extracellular ATP concentration. Changes in liver ecto-ATPase in estrogen-induced cholestasis were examined in male rats receiving 17 alpha-ethinylestradiol (E groups) for 1, 3, or 5 days (5 mg/kg/day, sc) and compared with changes in rats subjected to obstructive cholestasis (O groups) for 1, 3, or 8 days. Activity of ecto-ATPase, protein mass in canalicular membranes and bile (estimated by Western blotting), steady state mRNA levels (by Northern blotting), and cellular and acinar distributions of the enzyme (histochemistry and immunocytochemistry) were assessed in these groups. Activity of ecto-ATPase, protein mass in isolated canalicular membranes, and enzyme mRNA levels were significantly increased in E group rats as compared with controls. In contrast, these parameters were markedly decreased in O group rats, and the enzyme protein was undetectable in bile. The ecto-ATPase histochemical reaction was markedly increased in the canalicular membrane of E group rats, extending from acinar zone 2 to zone 1,whereas it decreased in the O group. The ecto-ATPase immunocytochemical reaction was present in the canalicular membrane and pericanalicular vesicles in control and E group hepatocytes, but it decreased in obstructive cholestasis and was localized only to the canalicular membrane. Thus, significant changes in liver ecto-ATPase were apparent in 17 alpha-ethinylestradiol-induced cholestasis that were opposite to those observed in obstructive cholestasis. Assuming that the alterations observed in obstructive cholestasis are the result of the cholestatic phenomenon, we conclude that changes in ecto-ATPase in 17 alpha-ethinylestradiol-treated rats might be either primary events or part of an adaptive response in 17 alpha-ethinylestradiol-induced cholestasis.
dc.fechaingreso.objetodigital2024-05-06
dc.format.extent13 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1067/mlc.2000.108151
dc.identifier.issn0022-2143
dc.identifier.pubmedidMEDLINE:10945241
dc.identifier.urihttps://doi.org/10.1067/mlc.2000.108151
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77033
dc.identifier.wosidWOS:000088645900007
dc.information.autorucMedicina;Accatino L;S/I;99016
dc.information.autorucMedicina;Arrese M;S/I;76095
dc.information.autorucMedicina;Chianale J;S/I;99780
dc.issue.numero2
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final137
dc.pagina.inicio125
dc.publisherMOSBY, INC
dc.revistaJOURNAL OF LABORATORY AND CLINICAL MEDICINE
dc.rightsacceso restringido
dc.subjectPLASMA-MEMBRANE
dc.subjectNUCLEOSIDE TRANSPORTER
dc.subjectALKALINE-PHOSPHATASE
dc.subjectBILIARY OBSTRUCTION
dc.subjectDOWN-REGULATION
dc.subjectBILE SECRETION
dc.subjectECTO-ATPASE
dc.subjectLIVER
dc.subjectHEPATOCYTES
dc.subjectPROTEINS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDifferential expression of canalicular membrane Ca2+/Mg2+-ecto-ATPase in estrogen-induced and obstructive cholestasis in the rat
dc.typeartículo
dc.volumen136
sipa.codpersvinculados99016
sipa.codpersvinculados76095
sipa.codpersvinculados99780
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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