Sonic hedgehog is basolaterally sorted from the TGN and transcytosed to the apical domain involving Dispatched-1 at Rab11-ARE

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dc.contributor.authorSandoval, Lisette
dc.contributor.authorLabarca, Mariana
dc.contributor.authorRetamal, Claudio
dc.contributor.authorSanchez, Paula
dc.contributor.authorLarrain, Juan
dc.contributor.authorGonzalez, Alfonso
dc.date.accessioned2025-01-20T21:01:02Z
dc.date.available2025-01-20T21:01:02Z
dc.date.issued2022
dc.description.abstractHedgehog proteins (Hhs) secretion from apical and/or basolateral domains occurs in different epithelial cells impacting development and tissue homeostasis. Palmitoylation and cholesteroylation attach Hhs to membranes, and Dispatched-1 (Disp-1) promotes their release. How these lipidated proteins are handled by the complex secretory and endocytic pathways of polarized epithelial cells remains unknown. We show that polarized Madin-Darby canine kidney cells address newly synthesized sonic hedgehog (Shh) from the TGN to the basolateral cell surface and then to the apical domain through a transcytosis pathway that includes Rab11-apical recycling endosomes (Rab11-ARE). Both palmitoylation and cholesteroylation contribute to this sorting behavior, otherwise Shh lacking these lipid modifications is secreted unpolarized. Disp-1 mediates first basolateral secretion from the TGN and then transcytosis from Rab11-ARE. At the steady state, Shh predominates apically and can be basolaterally transcytosed. This Shh trafficking provides several steps for regulation and variation in different epithelia, subordinating the apical to the basolateral secretion.
dc.fuente.origenWOS
dc.identifier.doi10.3389/fcell.2022.833175
dc.identifier.issn2296-634X
dc.identifier.urihttps://doi.org/10.3389/fcell.2022.833175
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/92809
dc.identifier.wosidWOS:000901808500001
dc.language.isoen
dc.revistaFrontiers in cell and developmental biology
dc.rightsacceso restringido
dc.subjecthedgehog
dc.subjectpolarity
dc.subjecttrafficking
dc.subjectDispatched
dc.subjectRab11-ARE
dc.subjecttranscytosis
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSonic hedgehog is basolaterally sorted from the TGN and transcytosed to the apical domain involving Dispatched-1 at Rab11-ARE
dc.typeartículo
dc.volumen10
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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