Identification of the interleukin-8 ( CXCL-8) pathway in feline oral squamous cell carcinoma - A pilot study

Simple item page
dc.contributor.authorAckerman, Leah H.
dc.contributor.authorSouza, Carlos H. de Mello
dc.contributor.authorCortes-Hinojosa, Galaxia
dc.contributor.authorSalute, Marc E.
dc.contributor.authorStephen, Alexa A.
dc.contributor.authorAnthony, Elizabeth
dc.contributor.authorShiomitsu, Keijiro
dc.contributor.authorMilner, Rowan J.
dc.date.accessioned2025-01-20T21:01:29Z
dc.date.available2025-01-20T21:01:29Z
dc.date.issued2022
dc.description.abstractThe purpose of this pilot study was to detect the presence of interleukin-8 (IL-8) and the potential downstream effects of IL-8 receptor activation in 2 previously characterized feline oral squamous cell carcinoma cell lines ( SCCF1 and SCCF2). Interleukin-8 messenger RNA (mRNA) was initially detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). A previously validated and commercially available enzyme-linked immunosorbent assay (ELISA) test was used to measure IL-8 production in the supernatant of the 2 cell lines. Western blot was used to detect phosphorylation of proteins (AKT, ERK1/2, JAK2, STAT3, and Src), known to be downstream of interleukin-8 receptor activation. The IL-8 receptor-specific antagonists, Reparixin and SCH527123, were used to identify effects on phosphorylation of these proteins. Interleukin-8 mRNA and protein were detected in both SCCF1 and SCCF2 by RT-PCR and ELISA, respectively. Phosphorylation of ERK1/2, STAT3, and Src was detected in both cell lines. Inhibition of the IL- 8 receptor led to a decrease in phosphorylation of Src, but not ERK1/2 or STAT3. In conclusion, feline squamous cell carcinoma cell lines can produce IL-8. Phosphorylation of Src seems, at least in part, a consequence of IL-8 receptor activation. The phosphorylation of ERK1/2 and STAT3, although present, seems independent of IL-8 receptor activation. Due to its potential effects on the tumor microenvironment, in addition to its autocrine effects on Src phosphorylation, the inhibition of the IL-8 receptor may become a beneficial therapeutic tool. Evaluation of the presence of both IL- 8 and Src in many cases should elucidate their importance.
dc.fuente.origenWOS
dc.identifier.eissn1928-9022
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/92895
dc.identifier.wosidWOS:000880460400002
dc.issue.numero1
dc.language.isoen
dc.pagina.final19
dc.pagina.inicio13
dc.revistaCanadian journal of veterinary research-revue canadienne de recherche veterinaire
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleIdentification of the interleukin-8 ( CXCL-8) pathway in feline oral squamous cell carcinoma - A pilot study
dc.typeartículo
dc.volumen86
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas