Down-regulation of the Na+/taurocholate cotransporting polypeptide during pregnancy in the rat

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dc.contributor.authorArrese, M
dc.contributor.authorTraumer, M
dc.contributor.authorAnanthanarayanan, M
dc.contributor.authorPizarro, M
dc.contributor.authorSolis, N
dc.contributor.authorAccatino, L
dc.contributor.authorSoroka, C
dc.contributor.authorBoyer, JL
dc.contributor.authorKarpen, SJ
dc.contributor.authorMiquel, JF
dc.contributor.authorSuchy, FJ
dc.date.accessioned2024-01-10T13:47:43Z
dc.date.available2024-01-10T13:47:43Z
dc.date.issued2003
dc.description.abstractBackground: Experimental studies have shown decreased bile acid (BA) uptake and reduced excretion of cholephilic compounds in pregnant rodents.
dc.description.abstractAim: To assess the expression and function of the main BA importer, the Na+/taurocholate cotransporting polypeptide (Ntcp) in pregnant rats.
dc.description.abstractMethods: BA uptake and Ntcp expression were studied in control and timed-pregnant rats in late gestation. Ntcp protein, messenger RNA (mRNA) expression, and Ntcp tissue localization were determined by Northern blotting, Western analysis, and tissue immunofluorescence. The activity of three transactivators of the Ntcp promoter: hepatocyte nuclear factor 1-alpha (HNF1-alpha), nuclear receptor heterodimer retinoid X receptor: retinoid acid receptor (RXR:RAR) and signal transducer and activator of transcription 5 (Stat5) was assessed using gel electrophoretic mobility shift assays.
dc.description.abstractResults: A significantly reduced BA uptake and decreased Ntcp mRNA levels (-40%) and protein mass (-60%) was observed in pregnant rats. Nuclear extracts from pregnant rats showed a marked decrease of HNF1-alpha and RXR:RAR binding activities by -80 and -40% of basal activity, respectively. In contrast, binding activity of Stat-5 was increased by 50% in nuclear extracts from pregnant rats.
dc.description.abstractConclusions: Pregnancy is associated with reduced Ntcp expression and function in the rat. Our findings suggest that Ntcp down-regulation during pregnancy occurs primarily at the transcriptional level. (C) 2002 European Association for the Study of the Liver. Published by Elsevier Science B.V. All rights reserved.
dc.description.funderNICHD NIH HHS
dc.description.funderNIDDK NIH HHS
dc.description.funderNIDDK NIH HHS
dc.fechaingreso.objetodigital16-04-2024
dc.format.extent8 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/S0168-8278(02)00379-3
dc.identifier.eissn1600-0641
dc.identifier.issn0168-8278
dc.identifier.pubmedidMEDLINE:11804680
dc.identifier.pubmedidMEDLINE:12547402
dc.identifier.urihttps://doi.org/10.1016/S0168-8278(02)00379-3
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79297
dc.identifier.wosidWOS:000180892900003
dc.information.autorucMedicina;Accatino L;S/I;99016
dc.information.autorucMedicina;Arrese M;S/I;76095
dc.information.autorucMedicina;Miquel J;S/I;72216
dc.issue.numero2
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final155
dc.pagina.inicio148
dc.publisherELSEVIER SCIENCE BV
dc.revistaJOURNAL OF HEPATOLOGY
dc.rightsacceso restringido
dc.subjectbile acid transport
dc.subjecttransporters
dc.subjectNa+/taurocholate cotransporting polypeptide
dc.subjectSALT EXPORT PUMP
dc.subjectBILE-ACID
dc.subjectINTRAHEPATIC CHOLESTASIS
dc.subjectNUCLEAR RECEPTOR
dc.subjectTRANSPORT
dc.subjectLIVER
dc.subjectMECHANISMS
dc.subjectEXPRESSION
dc.subjectPROLACTIN
dc.subjectHEPATOCYTES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDown-regulation of the Na+/taurocholate cotransporting polypeptide during pregnancy in the rat
dc.typeartículo
dc.volumen38
sipa.codpersvinculados99016
sipa.codpersvinculados76095
sipa.codpersvinculados72216
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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