<i>In vitro</i> culture of <i>Quillaja saponaria</i> Mol. (Soap-bark tree), Rosaceae

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dc.contributor.authorJordan, M
dc.contributor.authorRoveraro, C
dc.date.accessioned2025-01-21T01:08:04Z
dc.date.available2025-01-21T01:08:04Z
dc.date.issued2004
dc.description.abstractNodal sections of Quillaja saponaria, 'Quillay', including the proximal part of leaf laminae from 2-year old trees, as well as polynodal sections (3-4 axillary buds), were cultivated in MS-medium (MURASHIGE and SKOOG (1962) in the presence of various cytokinins, especially high levels of benzyladenine (BA) or thidiazuron (TDZ). This initiated profuse branching of the axillary bud and the induction of many new shoots and also callus formation in both explant types. From a single polynodal explant, more than 200 new multiple shoots developed in a period of 34 months. Proliferation of new shoots occurred mostly under the preexisting axillary bud(s) of the section and at the proximal end of the petiole with its intact leaf attached. Shoot clusters or isolated shoots elongated in subculture in the presence of MS-medium without growth regulators or formed roots when supplemented with indolebutyric acid (IBA) within a period of 30-60 days, leading to plantlets. Root formation occurred in 1/2 strength MS-medium with added 1 % sucrose and 0.6, 1.2 and 5.0 mg l(-1) IBA, as single hormone. Roots did not form in lower IBA levels (0.1 mg l(-1)) while the addition of phloroglucinol or thiamine did not enhance rhizogenesis besides the IBA. Despite the strong shoot initiation occurring in both nodal and polynodal explants, morphogenesis was not observed in leaf explant sections, and callus derived from nodal sections, formed green globular structures only without further differentiation.
dc.fuente.origenWOS
dc.identifier.issn1611-4426
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96421
dc.identifier.wosidWOS:000222587500005
dc.issue.numero2
dc.language.isoen
dc.pagina.final78
dc.pagina.inicio73
dc.revistaEuropean journal of horticultural science
dc.rightsacceso restringido
dc.subjectQuillaja saponaria
dc.subjectsoap-bark tree
dc.subjectin vitro propagation
dc.subjectorganogenesis
dc.subject.ods15 Life on Land
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.title<i>In vitro</i> culture of <i>Quillaja saponaria</i> Mol. (Soap-bark tree), Rosaceae
dc.typeartículo
dc.volumen69
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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