Transforming growth factor beta 1 is involved in the inhibitory effect of high D-glucose on human equilibrative nucleosidie transporter 1 in human fetal endothelium

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dc.catalogadorpau
dc.contributor.authorVega Pizarro, José Luis Eduardo
dc.contributor.authorFarías Jofré, Marcelo Enrique
dc.contributor.authorPuebla Aracena, Carlos Alberto
dc.contributor.authorGonzález Ortiz, Marcelo Andrés
dc.contributor.authorCasanello Toledo, Paola Cecilia
dc.contributor.authorSobrevía Luarte, Luis Alberto
dc.date.accessioned2023-07-21T17:46:18Z
dc.date.available2023-07-21T17:46:18Z
dc.date.issued2008
dc.description.abstractObjectives: High D-glucose (25 mM) reduces adenosine transport via the human equilibrative nucleoside transporters 1 (hENT1) in human umbilical vein endothelial cells (HUVEC). Interestingly, high D-glucose also increases the release of TGF-b1 from primary cultures of HUVEC leading to altered transport of L-arginine and synthesis of nitric oxide (NO), most likely due to activation of type II TGF-b1 receptors (TbRII). Since NO is involved in the repression of SLC29A1 gene expression in HUVEC, it is likely that D-glucose effect on hENT1 mediated adenosine transport involves TGF-b1 and activation of TbRII in this cell type. Therefore, we have studied whether TGF-b1 is involved in theinhibitory effect of high D-glucose on hENT1 in HUVEC. Methods: Cells were exposed to D-glucose (5-25 mM; 6-24 h) and/or TGF-b1 (0.1-10 ng/ml, 1-24 h) and [3H]adenosine transport (4 mCi/ml, 20 s, 22 C) was measured in absence or presence of nitrobenzylmercapto purine ribose (NBMPR, 1 mM, ENT1 inhibitor). These assays were performed in cells transduced with an adenovirus to induce expression of a truncated form of TbRII (Ad-tTbRII) or with a control adenovirus (Ad-control, empty vector). Results: In cells exposed to normal D-glucose (5 mM), TGF-b1 inhibited hENT1-mediated adenosine transport (~60%), an effect that was dose-dependent reaching a maximal inhibition at 2 ng/ml. The TGF-b1 effect was time-dependent with a significant inhibition of hENT1-mediated adenosine transport at 3 h of incubation, an effect that sustained for further 21 h. The effect of TGF-b1 was absent in cells transduced with Ad-tTbRII. In cells in high D-glucose (25 mM), hENT1-mediated adenosine transport was decreased compared with cells in normal D-glucose. This effect of high D-glucose was blocked in cells transduced with Ad-tTbRII, but it was unaltered in cells transduced with Adcontrol. Conclusion: These results suggest that TGF-b1 could mediate the inhibitory effect of high D-glucose on hENT1 activity by the activation of type II receptor for TGF-b1 in primary cultures of HUVEC.FONDECYT 1070865/1080534/7070249 (Chile), J.L. Vega, C. Puebla and M. González hold a CONICYT PhD fellowships. M. Farías holds CONICYT and PUC-School of Medicine PhD fellowships.
dc.format.extent1 página
dc.fuente.origenORCID
dc.identifier.doi10.1016/j.placenta.2008.06.002
dc.identifier.issn0143-4004
dc.identifier.urihttps://doi.org/10.1016/j.placenta.2008.06.002
dc.identifier.urihttps://publons.com/wos-op/publon/18766743/
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/74288
dc.information.autorucFacultad de Ciencias Biológicas ; Vega Pizarro, José Luis Eduardo ; 0000-0001-9333-8729 ; 165832
dc.information.autorucEscuela de Medicina ; Farías Jofré, Marcelo Enrique ; 0000-0003-0473-2295 ; 12286
dc.information.autorucFacultad de Ciencias Biológicas ; Puebla Aracena, Carlos Alberto ; 0000-0001-6648-2135 ; 124965
dc.information.autorucFacultad de Ciencias Biológicas ; González Ortiz, Marcelo Andrés ; 0000-0002-0521-3633 ; 158187
dc.information.autorucEscuela de Medicina ; Casanello Toledo, Paola Cecilia ; 0000-0002-2355-1476 ; 146772
dc.information.autorucEscuela de Medicina ; Sobrevía Luarte, Luis Alberto ; 0000-0001-5802-2243 ; 1002656
dc.issue.numero8
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.finalA18
dc.pagina.inicioA18
dc.relation.ispartofMeeting of the International-Federation-of-Placental-Associations (14° ; 2008 ; Seggau castle, Austria)
dc.revistaPlacentaes_ES
dc.rightsacceso restringido
dc.titleTransforming growth factor beta 1 is involved in the inhibitory effect of high D-glucose on human equilibrative nucleosidie transporter 1 in human fetal endotheliumes_ES
dc.typecomunicación de congreso
dc.volumen29
sipa.codpersvinculados165832
sipa.codpersvinculados12286
sipa.codpersvinculados124965
sipa.codpersvinculados158187
sipa.codpersvinculados146772
sipa.codpersvinculados1002656
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