Connexin hemichannel composition determines the FGF-1-induced membrane permeability and free [Ca2+](i) responses

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dc.contributor.authorSchalper Casanova, Kurt Alex
dc.contributor.authorPalacios Prado, Nicolás
dc.contributor.authorRetamal, Mauricio A.
dc.contributor.authorShoji Sánchez, Kenji Fabricio
dc.contributor.authorMartinez, Agustin D.
dc.contributor.authorSáez, Juan Carlos
dc.date.accessioned2024-01-10T12:04:44Z
dc.date.available2024-01-10T12:04:44Z
dc.date.issued2008
dc.description.abstractCell surface hemichannels (HCs) composed of different connexin (Cx) types are present in diverse cells and their possible role on FGF-1-induced cellular responses remains unknown. Here, we show that FGF-1 transiently (4-14 h, maximal at 7 h) increases the membrane permeability through HCs in HeLa cells expressing Cx43 or Cx45 under physiological extracellular Ca2+/Mg2+ concentrations. The effect does not occur in HeLa cells expressing HCs constituted of Cx26 or Cx43 with its C-terminus truncated at aa 257, or in parental nontransfected HeLa cells. The increase in membrane permeability is associated with a rise in HC levels at the cell surface and a proportional increase in HC unitary events. The response requires an early intracellular free Ca2+ concentration increase, activation of a p38 MAP kinase-dependent pathway, and a regulatory site of Cx subunit C-terminus. The FGF-1-induced rise in membrane permeability is also associated with a late increase in intracellular free Ca2+ concentration, suggesting that responsive HCs allow Ca2+ influx. The cell density of Cx26 and Cx43 HeLa transfectants cultured in serum-free medium was differentially affected by FGF-1. Thus, the FGF-1-induced cell permeabilization and derived consequences depend on the Cx composition of HCs.
dc.description.funderFondo Nacional de Desarrollo Cientifico y Technologico
dc.description.funderComision Nacional de Investigacion Cientifico y Technologico
dc.description.funderProyecto Anillo de Ciencia Y Tecnologia
dc.fechaingreso.objetodigital2024-04-30
dc.format.extent13 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1091/mbc.E07-12-1240
dc.identifier.eissn1939-4586
dc.identifier.issn1059-1524
dc.identifier.pubmedidMEDLINE:18495870
dc.identifier.urihttps://doi.org/10.1091/mbc.E07-12-1240
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75870
dc.identifier.wosidWOS:000259160400030
dc.information.autorucCiencias Biológicas;Palacios-Prado N;S/I;124928
dc.information.autorucCiencias Biológicas;Retamal M;S/I;127950
dc.information.autorucMedicina;Schalper K;S/I;151783
dc.information.autorucCiencias Biológicas;Shoji K;S/I;134136
dc.information.autorucCiencias Biológicas;Sáez J;S/I;99913
dc.issue.numero8
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final3513
dc.pagina.inicio3501
dc.publisherAMER SOC CELL BIOLOGY
dc.revistaMOLECULAR BIOLOGY OF THE CELL
dc.rightsacceso restringido
dc.subjectGAP-JUNCTION CHANNELS
dc.subjectACTIVATED PROTEIN-KINASE
dc.subjectPOSSIBLE INVOLVEMENT
dc.subjectCA2+ ELEVATION
dc.subjectGROWTH
dc.subjectCALCIUM
dc.subjectCELLS
dc.subjectPHOSPHORYLATION
dc.subjectCOMMUNICATION
dc.subjectASTROCYTES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleConnexin hemichannel composition determines the FGF-1-induced membrane permeability and free [Ca2+](i) responses
dc.typeartículo
dc.volumen19
sipa.codpersvinculados124928
sipa.codpersvinculados127950
sipa.codpersvinculados151783
sipa.codpersvinculados134136
sipa.codpersvinculados99913
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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